Evolutionary relationships of SctU proteins. The yellow star indicates the position of the P. syringae pv phaseolicola 1448a Hrc
U. A. The phylogram of 192 SctU sequences with the eight main families named according to Troisfontaines & Cornelis (2005) , while the flagellum proteins are depicted in black. The T3SS family encompasing the β-rhizobium Cupriavidus taiwanensis and of Burkholderia cenocepacia group is indicated here with a light purple color (marked as β-Rhc). Branches corresponding to partitions reproduced in less than 50% bootstrap replicates are collapsed. There were a total of 686 positions in the final dataset. Phylogenetic analyses were conducted in MEGA4 . B. The Rhc T3SS clade as derived from the phylogram in A, groups the P. syringae Hrc
U sequences close to the Rhc
U protein of the Rhizobium sp. NGR234 T3SS-2. The values at the nodes are the bootstrap percentages out of 1000 replicates. The locus numbers or the protein accession number of each sequence is indicated.