Figure 1

Evolutionary relationships of SctU proteins. The yellow star indicates the position of the P. syringae pv phaseolicola 1448a Hrc _II U. A. The phylogram of 192 SctU sequences with the eight main families named according to Troisfontaines & Cornelis (2005) [8], while the flagellum proteins are depicted in black. The T3SS family encompasing the β-rhizobium Cupriavidus taiwanensis and of Burkholderia cenocepacia group is indicated here with a light purple color (marked as β-Rhc). Branches corresponding to partitions reproduced in less than 50% bootstrap replicates are collapsed. There were a total of 686 positions in the final dataset. Phylogenetic analyses were conducted in MEGA4 [21]. B. The Rhc T3SS clade as derived from the phylogram in A, groups the P. syringae Hrc _II U sequences close to the Rhc _II U protein of the Rhizobium sp. NGR234 T3SS-2. The values at the nodes are the bootstrap percentages out of 1000 replicates. The locus numbers or the protein accession number of each sequence is indicated.