Anti-chlamydial properties of 405 nm irradiance. (A-C) HeLa cells were infected with C. trachomatis serovar E at a MOI of 5 without exposure to photodiodes. (D-F) Infected cells were exposed to 405 nm LEDs at 20 J/cm2 promptly after infection to evaluate anti-chlamydial effects during an acute chlamydial infection. Cells were fixed and stained with dapi (blue) (B and E) and anti-chlamydial (green) (C and F) antibody 48 hours post-infection. Bar = 10μm.