Skip to main content

Advertisement

Table 1 Strains and plasmids used in this study

From: Opposing roles of σB and σB-controlled SpoVG in the global regulation of esxA in Staphylococcus aureus

Strain or plasmid Relevant genotype; phenotype Reference or source
S. aureus   
Newman Clinical isolate, ATCC 25904, natural saeS constitutive mutant [21, 22]
BS304 Newman ΔesxA, markerless deletion This study
SM148 Newman Δ(yabJ-spoVG)::erm(B); Emr [8]
IK184 Newman Δ(rsbUVW-sigB)::erm(B); Emr [23]
MS64 Newman sigB1(Am); Tcr [24]
SM99 Newman ΔarlR::cat; Cmr [9]
BS310 Newman Δagr::ermB; Emr This study
KS186 Newman Δagr::tet(M); Tcr [25]
BS309 Newman ΔsarA::ermB; Emr This study
LR15 Newman ΔsarA::tet(L); Tcr L. Reutimann
BB1002 Newman mec, MRSA derivative, Mcr [26]
BS307 BB1002 ΔesxA, markerless deletion This study
NM143 Newman GISA derivative, in vitro selected mutant; Ter [27]
BS308 NM143 ΔesxA, markerless deletion This study
E. coli   
DH5α F-Φ80d/acZΔM15 recA1 Invitrogen
Plasmids   
pKOR1 E. coli-S. aureus shuttle vector for markerless deletions using the counter selection system [28]
pAC7 Expression plasmid containing the PBAD promoter and the araC gene; Cmr [29]
pAC7-sigB pAC7 with a 0.75 kb fragment containing the gene sigB from S. aureus Col; Cmr [30]
pBus1 E. coli-S. aureus shuttle plasmid with multicloning site from pBluescript II SK (Stratagene) and the rrnT14 terminator sequence from pLL2443; Tcr [31]
pyabJ pBus1 containing a bacA promoter-yabJ ORF fusion construct; Tcr [10]
pspoVG pBus1 containing a bacA promoter-spoVG ORF fusion construct; Tcr [10]
pyabJspoVG pBus1 containing a bacA promoter-yabJ-spoVG operon fusion construct; Tcr [10]
pSP-luc+ Firefly luciferase casette vector; Apr Promega
pesxAp-luc+ pBus1 containing an esxAp-luc+ fusion fragment; Tcr This study
pesxApΔσA-luc+ pesxAp-luc+ with deletion of the σA promoter This study
pesxApΔσB-luc+ pesxAp-luc+ with deletion of the σB promoter This study
pSB40N Promoter probe plasmid; Apr [29]
pasp23p pSB40N with a 0.6 kb fragment covering the asp23 promoter region fused to the reporter gene lacZα; Apr [30]
pesxAp pSB40N with a 0.5 kb fragment covering the esxA promoter region fused to the reporter gene lacZα; Apr This study
pyabJp pSB40N with a 0.4 kb fragment covering the yabJ promoter region fused to the reporter gene lacZα; Apr This study
pSTM07 pSB40N with a 0.37 kb fragment covering the capA promoter region fused to the reporter gene lacZα; Apr [9]
  1. aAbbreviations are as follows: Apr, ampicillin resistant; Cmr, chloramphenicol resistant; Emr, erythromycin resistant; Mcr, methicillin resistant; Tcr, tetracycline resistant; Ter, teicoplanin resistant.