Newman
|
Clinical isolate, ATCC 25904, natural saeS constitutive mutant
|
[21, 22]
|
BS304
|
Newman ΔesxA, markerless deletion
|
This study
|
SM148
|
Newman Δ(yabJ-spoVG)::erm(B); Emr
|
[8]
|
IK184
|
Newman Δ(rsbUVW-sigB)::erm(B); Emr
|
[23]
|
MS64
|
Newman sigB1(Am); Tcr
|
[24]
|
SM99
|
Newman ΔarlR::cat; Cmr
|
[9]
|
BS310
|
Newman Δagr::ermB; Emr
|
This study
|
KS186
|
Newman Δagr::tet(M); Tcr
|
[25]
|
BS309
|
Newman ΔsarA::ermB; Emr
|
This study
|
LR15
|
Newman ΔsarA::tet(L); Tcr
|
L. Reutimann
|
BB1002
|
Newman mec, MRSA derivative, Mcr
|
[26]
|
BS307
|
BB1002 ΔesxA, markerless deletion
|
This study
|
NM143
|
Newman GISA derivative, in vitro selected mutant; Ter
|
[27]
|
BS308
|
NM143 ΔesxA, markerless deletion
|
This study
|
E. coli
| | |
DH5α
|
F-Φ80d/acZΔM15 recA1
|
Invitrogen
|
Plasmids
| | |
pKOR1
|
E. coli-S. aureus shuttle vector for markerless deletions using the counter selection system
|
[28]
|
pAC7
|
Expression plasmid containing the PBAD promoter and the araC gene; Cmr
|
[29]
|
pAC7-sigB
|
pAC7 with a 0.75 kb fragment containing the gene sigB from S. aureus Col; Cmr
|
[30]
|
pBus1
|
E. coli-S. aureus shuttle plasmid with multicloning site from pBluescript II SK (Stratagene) and the rrnT14 terminator sequence from pLL2443; Tcr
|
[31]
|
pyabJ
|
pBus1 containing a bacA promoter-yabJ ORF fusion construct; Tcr
|
[10]
|
pspoVG
|
pBus1 containing a bacA promoter-spoVG ORF fusion construct; Tcr
|
[10]
|
pyabJspoVG
|
pBus1 containing a bacA promoter-yabJ-spoVG operon fusion construct; Tcr
|
[10]
|
pSP-luc+
|
Firefly luciferase casette vector; Apr
|
Promega
|
pesxAp-luc+
|
pBus1 containing an esxAp-luc+ fusion fragment; Tcr
|
This study
|
pesxApΔσA-luc+
|
pesxAp-luc+ with deletion of the σA promoter
|
This study
|
pesxApΔσB-luc+
|
pesxAp-luc+ with deletion of the σB promoter
|
This study
|
pSB40N
|
Promoter probe plasmid; Apr
|
[29]
|
pasp23p
|
pSB40N with a 0.6 kb fragment covering the asp23 promoter region fused to the reporter gene lacZα; Apr
|
[30]
|
pesxAp
|
pSB40N with a 0.5 kb fragment covering the esxA promoter region fused to the reporter gene lacZα; Apr
|
This study
|
pyabJp
|
pSB40N with a 0.4 kb fragment covering the yabJ promoter region fused to the reporter gene lacZα; Apr
|
This study
|
pSTM07
|
pSB40N with a 0.37 kb fragment covering the capA promoter region fused to the reporter gene lacZα; Apr
|
[9]
|