Production of fungal and bacterial growth modulating secondary metabolites is widespread among mycorrhiza-associated streptomycetes

Table 2 Chemical diversity of Norway spruce mycorrhiza associated Streptomyces

Strain	Medium	Substance based on UV–vis	Measured [M + H]+	Theoretical [M + H]+	Confirmed
AcM9	SGG	Unknown	180,1	n. a.	n. a.
AcM11	OM	Cycloheximide	282,1	282,169825	Yes
AcM11	OM	Actiphenol	276,1	276,123525	Yes
AcM11	OM	Acta 2930 B1	1007,5	1008,507825	No
AcM11	OM	Ferulic acid	195	195,065735	Yes
AcM11	OM	Unknown	292	n. a.	n. a.
AcM11	OM	Unknown	407	n. a.	n. a.
AcM11	OM	Unknown	387	n. a.	n. a.
AcM20	SGG	Unknown	180,1	n. a.	n. a.
AcM20	OM	Unknown	298	n. a.	n. a.
AcM29	SGG	Desferrioxamine B	561,5	561,691825	Yes
AcM29	SGG	Unknown	180	n. a.	n. a.
AcM29	SGG	Unknown	340	n. a.	n. a.
AcM29	SGG	Unknown	523	n. a.	n. a.
AcM29	SGG	Unknown	482	n. a.	n. a.
AcM29	OM	Ferulic acid	195,1	195,065735	Yes
AcM29	OM	Unknown	298,3	n. a.	n. a.
AcM29	OM	Unknown	477,3	n. a.	n. a.
AcM29	OM	Unknown	151,1	n. a.	n. a.
AcM29	OM	Unknown	217,2	n. a.	n. a.
AcM30	SGG	Anthranilic acid	138	138,054825	Yes
AcM30	SGG	Silvalactam	637,6	637,427825	Yes

The metabolite spectra of five selected Streptomyces strains were investigated. The bacteria were grown on oat meal (OM) and starch-glucose-glycerol (SGG) media. The substances were identified based on their UV–vis spectra and on their molecular mass, determined by ESI-LC-MS. The term “Confirmed” refers to verification of compound identity by comparison with the purified substance. Apart from the listed metabolites the Streptomyces strains produced the following numbers of other peaks: AcM9, five; AcM11, nine; AcM20, eight; AcM29, eleven; AcM30, six.

ISSN: 1471-2180