Figure 4

The attachment and infection of EV71 to RD cells are affected by sialic acid specific lectin treatment. Cells were preincubated with MAA (maackia amurensis) or SNA (sambucus nigra) followed by infection with EV71 MP4. The bound EV71 was analyzed by ELISA and real-time PCR, and the subsequent replication of EV71 in RD cells was detected by real-time PCR analysis. The binding of virus to RD cells treated with different concentrations of MAA was reduced by 19% and 45% measured by ELISA (A) and by 37% and 68% measured by real-time PCR (C). The replication of EV71 dropped 38% and 59% after MAA treatment measured by real-time PCR after 24 hours incubation (E). The virus binding of SNA treated cells reduced by 18% and 38% measured by ELISA (B), and by 28% and 45% measured by real-time PCR (D). The replication of EV71 dropped 30% and 58% after SNA treatment measured by RT-PCR after 24 hours incubation (F). **: P < 0.01; ***: P < 0.001 (two-tailed test). Each of the results was averaged from at least six independent assays.