Figure 4
From: Expression of sialic acids and other nonulosonic acids in Leptospira
 Leptospira interrogans  endogenously expresses N-acetylneuraminic acid (Neu5Ac). L. interrogans was grown in EMJH medium or in a chemically defined medium containing no exogenous sialic acid (this was confirmed by HPLC, not shown). Covalently bound Sias were released by mild acid hydrolysis and analyzed by DMB-derivatization and HPLC as described in previous figures and Materials and Methods. This experiment was performed twice with similar results.