Figure 3

The gene homologous to the bbk32 was not detected in N40D10/E9 strain by PCR and Southern hybridization. (A). Using the primers in Additional Table 1, complete genes bb0153, bb0184 (csrA), bb0219, bb0268, bb0383 (bmpA), bb0647 (bosR), bba25 (dbpB), bba68 (cspA), bbd14, bbd18, bbe22 (pncA), bbg02, bbh06 (cspZ), bbj09 (ospD), bbk17 (adeC), bbu06, and partial vlsE1 gene (using internal conserved primers) were amplified by PCR using B31 and N40D10/E9 strains genomic DNA as template. The bbk32 gene was amplified from B31 genomic DNA, however, PCR product was not detected in the N40D10/E9 strain. (B) Southern blot of EcoR1-digested genomic DNA of both strains (top) was hybridized with the probe prepared using the bbk32 PCR product from B31. An approximately 1.8 kb size fragment was detected only in B31, as expected, but not in the N40D10/E9 genomic DNA containing lane.