Figure 2From: Role of the small RNA RyhB in the Fur regulon in mediating the capsular polysaccharide biosynthesis and iron acquisition systems in Klebsiella pneumoniaeRyhB activates CPS biosynthesis. (A) Comparison of CPS levels in WT, ΔryhB, Δfur, and Δfur ΔryhB strains. Bacterial strains were grown in LB medium at 37°C with agitation. After 16 h of growth, the bacterial glucuronic acid content was determined. *, P < 0.001 compared with WT. (B) WT strains carrying the vector control (pETQ) or pETQ-ryhB were grown in LB with 100 μM IPTG to induce ryhB expression. *, P < 0.001 compared with WT strains carrying pETQ.Back to article page