BLI of B6(Cg)- Tyrc-2J /J mice infected intradermally with Yp lux+in the ear pinna. (A) Mice were inoculated with ~200 CFU and were imaged (ventral and dorsal sides) at the indicated hours post inoculation (hpi). Luminescence signal is reported as radiance (p/sec/cm2/sr) in a scale paired with a color bar shown next to the images. For 24 hpi (dorsal view), the window shows an image with signal at an individual radiance color scale with of Min = 1.11e4 and Max = 1.43e5. (B) Site of infection (right ear), superficial parotid right and left lymph nodes, spleen and liver (from one of the mice shown in A) imaged individually after dissection. An asterisk denotes the LN that drains the site of infection. Individual scales of radiance were used due to variability in signal (site of infection, Min = 1.89e4 Max = 8.97e4; lymph nodes, Min = 1.89e6 Max = 8.97e7; spleen and liver, Min = 5.25e5 Max = 2.34e7). Shown is a representative experiment.