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Table 3 Observed and predicted TRF lengths a

From: Diversity and dynamics of Archaea in an activated sludge wastewater treatment plant

Observed TRF lengths T-RFLP 2007-05-22 Predicted TRF lengths, clone library 2007-05-22
AluI %b RsaI %b AluI RsaI %c Identity
     84 80 1% Thaumarchaeota/Cluster 1.3
     126 608 1% Thermoplasmatales/Cluster B
     166 609 2% Thermoplasmatales/Cluster B
     180 80 4% Methanosaeta
183 100% 74 86% 188 80 48% Methanosaeta
   238 14% 188 242 21% Methanosaeta
     188 NDd 2% Methanosaeta
     189 264 1% ARC I
     359 262 1% Thaumarchaeota/Cluster 1.1b
     400 609 1% Thermoplasmatales/RCIII
     515 264 1% Methanocorpusculum
     551 609 10% Thermoplasmatales/RCIII
     ND 80 1% Methanosaeta
     ND 613 1% Thermoplasmatales/Cluster C
     ND ND 4% Methanosaeta
  1. a Observed and predicted TRF lengths from T-RFLP and clone library analysis of a sample from 2007-05-22. b Relative abundance based on total fluorescence. c Relative abundance based on frequency in clone library. d ND indicates cases where a TRF could not be predicted or where the predicted TRF was outside the detection range.