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Table 2 Effect of growth condition on intra- and extra-cellular iron concentrations and gene regulation

From: The role of MglA for adaptation to oxidative stress of Francisella tularensis LVS

Parameter tested Growth condition
  Aerobic Microaerobic
  LVS Δ mglA FUU301 LVS Δ mglA FUU301
Fe intraa 626 ± 27.2 661 ± 17.1 643 ± 24.5 893 ± 33.8 589 ± 21.9d 662 ± 20.5d
Fe extrab B.D.L.e 186 ± 20.5 64.5 ± 8.97 73.9 ± 19.3 327 ± 10.7d 165 ± 46.1
Gene regulationc
fslA 12.7 ± 0.64 2.51 ± 0.19f 10.6 ± 1.33 5.87 ± 0.71 4.93 ± 0.48 9.29 ± 1.19g
fslB 6.27 ± 0.39 0.83 ± 0.15f 5.6 ± 1.09 2.86 ± 0.43 1.87 ± 0.30 5.86 ± 0.30
fslC 5.96 ± 0.36 0.74 ± 0.15f 4.86 ± 0.68 2.61 ± 0.33 1.55 ± 0.28g 4.69 ± 0.26g
fslD 3.19 ± 0.23 0.97 ± 0.15f 3.52 ± 0.35 1.60 ± 0.23 2.40 ± 0.27g 3.73 ± 0.37g
fslE 0.82 ± 0.24 1.11 ± 0.15 1.55 ± 0.20h 1.04 ± 0.06 1.98 ± 0.14d 5.43 ± 1.20d
feoB 4.03 ± 0.29 1.37 ± 0.15f 4.95 ± 0.27 5.50 ± 0.41 4.33 ± 0.52 12.8 ± 3.77
katG 50.7 ± 8.62 110 ± 15.3h 116 ± 18.21h 79.1 ± 7.14 120 ± 19.3 135 ± 12.2i
iglC 390 ± 140 24.6 ± 5.37f 385 ± 58 685 ± 159 38.5 ± 15.9d 478 ± 120
mglA 16.5 ± 5.77 B.D.L. 384 ± 138h 63.7 ± 17 B.D.L. 637 ± 173g
  1. a The intracellular iron pool (ng/OD600 nm) of the strains after 18 h of growth
  2. b Iron (ng/ml) remaining in the culture medium after 18 h of growth
  3. c The expression of the genes was analyzed by quantitative real-time PCR. Results are expressed as RCN means ± SEM of results from four independent samples
  4. d P < 0.001 relative to LVS in the microaerobic condition
  5. e Below Detection Limit
  6. f P < 0.001 relative to LVS in the aerobic condition
  7. g P < 0.05 relative to LVS in the microaerobic condition
  8. h P < 0.05 relative to LVS in the aerobic condition
  9. i P < 0.01 relative to LVS in the microaerobic condition