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Table 2 Effect of growth condition on intra- and extra-cellular iron concentrations and gene regulation

From: The role of MglA for adaptation to oxidative stress of Francisella tularensis LVS

Parameter tested

Growth condition

 

Aerobic

Microaerobic

 

LVS

Δ mglA

FUU301

LVS

Δ mglA

FUU301

Fe intraa

626 ± 27.2

661 ± 17.1

643 ± 24.5

893 ± 33.8

589 ± 21.9d

662 ± 20.5d

Fe extrab

B.D.L.e

186 ± 20.5

64.5 ± 8.97

73.9 ± 19.3

327 ± 10.7d

165 ± 46.1

Gene regulationc

fslA

12.7 ± 0.64

2.51 ± 0.19f

10.6 ± 1.33

5.87 ± 0.71

4.93 ± 0.48

9.29 ± 1.19g

fslB

6.27 ± 0.39

0.83 ± 0.15f

5.6 ± 1.09

2.86 ± 0.43

1.87 ± 0.30

5.86 ± 0.30

fslC

5.96 ± 0.36

0.74 ± 0.15f

4.86 ± 0.68

2.61 ± 0.33

1.55 ± 0.28g

4.69 ± 0.26g

fslD

3.19 ± 0.23

0.97 ± 0.15f

3.52 ± 0.35

1.60 ± 0.23

2.40 ± 0.27g

3.73 ± 0.37g

fslE

0.82 ± 0.24

1.11 ± 0.15

1.55 ± 0.20h

1.04 ± 0.06

1.98 ± 0.14d

5.43 ± 1.20d

feoB

4.03 ± 0.29

1.37 ± 0.15f

4.95 ± 0.27

5.50 ± 0.41

4.33 ± 0.52

12.8 ± 3.77

katG

50.7 ± 8.62

110 ± 15.3h

116 ± 18.21h

79.1 ± 7.14

120 ± 19.3

135 ± 12.2i

iglC

390 ± 140

24.6 ± 5.37f

385 ± 58

685 ± 159

38.5 ± 15.9d

478 ± 120

mglA

16.5 ± 5.77

B.D.L.

384 ± 138h

63.7 ± 17

B.D.L.

637 ± 173g

  1. a The intracellular iron pool (ng/OD600 nm) of the strains after 18 h of growth
  2. b Iron (ng/ml) remaining in the culture medium after 18 h of growth
  3. c The expression of the genes was analyzed by quantitative real-time PCR. Results are expressed as RCN means ± SEM of results from four independent samples
  4. d P < 0.001 relative to LVS in the microaerobic condition
  5. e Below Detection Limit
  6. f P < 0.001 relative to LVS in the aerobic condition
  7. g P < 0.05 relative to LVS in the microaerobic condition
  8. h P < 0.05 relative to LVS in the aerobic condition
  9. i P < 0.01 relative to LVS in the microaerobic condition