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Figure 4 | BMC Microbiology

Figure 4

From: Variation of chemosensory receptor content of Campylobacter jejuni strains and modulation of receptor gene expression under different in vivo and in vitro growth conditions

Figure 4

Quantitative protein analysis of cellular Tlp1 levels. a.) Representative blot result from a Western blot performed using anti-Tlp1 sera. Samples are as follows for Tlp1 and Con; I). C. jejuni 11168-O maintained at room temperature in pond water; II). grown at 37°C; III). grown at 42°C. IV). C. jejuni 11168-GS maintained at room temperature in pond water; V). grown at 37°C; VI). grown at 42°C. VII). C. jejuni 81116 maintained at room temperature in pond water; VIII). grown at 37°C; IX). grown at 42°C. A single band was observed at ~75 kDa corresponding to the predicted size of Tlp1. The loading control shows the band (~30 kDa) that was used to ensure the same amount of protein was loaded in each well. b.) Quantitative densitometry analysis of Tlp1 protein detected by anti-Tlp1 sera. Average background subtracted band intensity was determined using QuantityOne software (Bio-Rad) from triplicate repeat anti-Tlp1 Western blots of C. jejuni 11168-O, 11168-GS and 81116 maintained at room temperature in pond water; grown at 37°C; grown at 42°C. Errors bars equal to 3x standard error of the mean (SEM).

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BMC Microbiology

ISSN: 1471-2180

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