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Figure 3 | BMC Microbiology

Figure 3

From: Identification and recombinant expression of anandamide hydrolyzing enzyme from Dictyostelium discoideum

Figure 3

CE-ES-MS analysis of anandamide hydrolysis by recombinant FAAH from both Dictyostelium and E.coli. (A) CE-ES-MS analysis of control reaction having anandamide alone in the reaction buffer without enzyme was analyzed. Negative ion mode product ion scan of mass [m/z 346.3]- corresponds to substrate anandamide. Inset figure is the structure of anandamide. (B) CE-ES-MS analysis of anandamide hydrolysis by recombinant HIS-FAAH purified from Dictyostelium. Negative ion mode product ion scan of mass [m/z 346.3]- corresponds to substrate anandamide and mass [m/z 303.5]- corresponds to hydrolyzed product arachidonic acid. Inset figures are the structure of anandamide and arachidonic acid. (C) CE-ES-MS analysis of anandamide hydrolysis of recombinant MBP-FAAH purified form E.coli. Negative ion mode product ion scan of mass [m/z 346.3]- corresponds to substrate anandamide and mass [m/z 303.5]- corresponds to hydrolyzed product arachidonic acid. Inset figures are the structure of anandamide and arachidonic acid.

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BMC Microbiology

ISSN: 1471-2180

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