Variation in endogenous oxidative stress in Escherichia coli natural isolates during growth in urine

BMC Microbiology

Table 2 Comparison of TBARS content of eight E. coli at both phases (exponential and stationary) of growth in pooled human urine and LB broth

	Urine exponential phase		Urine stationary phase		Urine exponential phase vs stationary phase
Strains	TBARS*	p**	TBARS	p	p
ABU83972	7.3 ± 1.0		4.4 ± 0.4		p = 0.014
CFT073	6.3 ± 0.8	p = 0.902	4.7 ± 0.8	p = 1.000	p = 0.450
ED1a	5.2 ± 1.1	p = 0.070	5.2 ± 0.8	p = 0.927	p = 1.000
536	5.1 ± 1.0	p = 0.048	4.1 ± 0.6	p = 1.000	p = 0.993
IAI1	4.3 ± 0.7	p = 0.002	4.6 ± 0.7	p = 1.000	p = 1.000
Sakai	3.9 ± 0.4	p = 0.001	4.2 ± 0.3	p = 1.000	p = 1.000
UIT89	3.8 ± 0.6	p = 0.001	3.9 ± 0.1	p = 0.997	p = 1.000
MG1655	2.6 ± 0.5	p < 0.0001	4.0 ± 1.0	p = 0.999	p = 0.880
	LB broth exponential phase		LB broth stationary phase		LB broth exponential phase vs stationary phase
Strains	TBARS	p	TBARS	p	p
ABU83972	6.4 ± 0.1		8.9 ± 1.6		p = 0.394
CFT073	5.9 ± 0.6	p = 0.993	6.5 ± 0.4	p = 0.458	p = 1.000
ED1a	4.9 ± 0.2	p = 0.492	6.8 ± 1.2	p = 0.581	p = 0.763
536	6.3 ± 1.7	p = 1.000	5.4 ± 1.9	p = 0.135	p = 0.998
IAI1	4.4 ± 0.3	p = 0.219	6.8 ± 0.1	p = 0.571	p = 0.465
Sakai	4.6 ± 0.7	p = 0.316	6.7 ± 1.1	p = 0.543	p = 0.635
UIT89	5.1 ± 0.1	p = 0.656	7.4 ± 0.4	p = 0.844	p = 0.540
MG1655	5.5 ± 0.1	p = 0.907	7.0 ± 0.1	p = 0.680	p = 0.942

* TBARS values are expressed in micromoles per 10¹¹ cells. The data shown are means (mean ± SD) of three independent experiments in different batches of urine and LB broth. Differences between means were evaluated for statistical significance using the Tukey's HSD (Honestly Significant Difference) test.
** p values of < 0.05 were considered significant. compared to ABU83972 under the same conditions.

ISSN: 1471-2180