Dominance of multidrug resistant CC271 clones in macrolide-resistant Streptococcus pneumoniae in Arizona

BMC Microbiology

Table 1 Oligonucleotides used in transposon detection

Transposon region	Oligo nucleotide	Sequence	Amplicon size (bp)	Transposon presumed present	*S. pneumoniae*population screened	Reference
int gene	int_for	GCGTGATTGTATCTCACT	1046	Tn916	Dual-positive, erm(B)-positive, mef(E)-positive	[29]
	int_rev	GACGCTCCTGTTGCTTCT				[29]
xis gene	xis_for	AAGCAGACTGAGATTCCTA	193	Tn916	Dual-positive, erm(B)-positive, mef(E)-positive	[29]
	xis rev	GCGTCCAATGTATCTATAA				[29]
tnpRgene	O21	CCAAGGAGCTAAAGAGGTCCC	1528	Tn917	Dual-positive, erm(B)-positive, mef(E)-positive	[29]
	O22	GTCCCGAGTCCCATGGAAGC				[29]
tnpA gene	O23	GCTTCCATGGGACTCGGGAC	2115	Tn917	Dual-positive, erm(B)-positive, mef(E)-positive	[29]
	O24	GCTCCCAATTAATAGGAGA				[29]
Spans insert of erm(B) elements in Tn916	J12^d	ATTCCCATTGAAGACGCAGAAGT	800	Tn3872	erm(B)-positive that are Tn916-positive	[34]
	J11^d	CTACCGCACTTCGTTTGGTGTAC	3600	Tn6002		[34]
			7900	Tn6003 or Tn1545
Junction of mega insert and Tn916	SG1	CTCACTGCACCAGAGGTGTA	1000	Tn2009 or Tn2010	Dual-positive and mef(E)-positivie that are Tn916-positive	[30]
	LTf	GCAGAGTATACCATTCACATCGAAGTTCCAC				30]
Junction of erm(B) element and Tn916	EB2	AGTAATGGTACTTAAATTGTTTAC	3300	Tn2010	Dual-positive that are Tn916-positive	[31]
	TN2^a	GAAGTA(G/C)AAGCTAAAGATGG				[32]

^a Modified from original to change melt temperature or incorporate degeneracies

ISSN: 1471-2180