Figure 6

Cytokine signaling for human iNos2 translation is dependent on the hypusine pathway during the infection of Plasmodium. Western Blot analysis was performed with equal amounts of protein (10 μg) extracted from the infected erythrocytic stages with transgenic schizonts from P. berghei ANKA strain 1) protein extract prepared from serum after infection with schizonts harbouring the expressed plasmodial DHS-shRNA or 2) the eIF-5A-siRNA expression construct; 3) P. berghei ANKA strain; 4) induced and 5) non- induced Jurkat cells; 6) Mono Mac 1 cells; 7) HeLa cells; M) Standard protein marker Roth, St. Leon, Germany. Detection of the iNos2 protein with a molecular size of 131 kDa was performed with a human anti-Nos2 antibody in a dilution of 1:1000.