Aspergillus flavus grown in peptone as the carbon source exhibits spore density- and peptone concentration-dependent aflatoxin biosynthesis

Table 1 Metabolites differ between high and low spore density cultures

Groups	Metabolites	R. T.	Relative contents	Relative contents	10⁴/10⁶
		(sec)	in 10⁴spores/ml*	in 10⁶spores/ml*	ratio
TCA cycle intermediates	Malic acid	587.5	0.0187 ± 0.0001	0.0098 ± 0.0027	1.91
	Fumaric acid	1349.0	0.0869 ± 0.0090	0.0509 ± 0.0043	1.71
	Succinic acid	269.8	0.0103 ± 0.0020	0.0043 ± 0.0016	2.4
Amino acids	Glycine	417.0	0.0066 ± 0.0028	0.0034 ± 0.0003	1.94
	Phenylalanine	713.7	0.1649 ± 0.0330	0.0084 ± 0.0007	19.63
	Proline	410.0	0.0552 ± 0.0179	0.0099 ± 0.0009	5.58
	Valine	331.5	0.0191 ± 0.00028	0.0088 ± 0.0006	2.17
	Tyrosine	964.1	0.0430 ± 0.0090	0.0242 ± 0.0027	1.78
	Isoleucine	404.0	0.0059 ± 0.0017	0.0042 ± 0.0003	1.4
Fatty acids	Palmitic acid	1054.5	0.3447 ± 0.037	0.2640 ± 0.011	1.31
	Stearic acid	1212.4	0.2218 ± 0.027	0.1402 ± 0.0133	1.58
	Oleic acid	1193.0	0.0833 ± 0.0024	0.0744 ± 0.0033	1.12
	Linoleic acid	1188.8	0.8959 ± 0.0671	0.6315 ± 0.0554	1.42
Nucleotides	Pyrimidine	449.0	0.0154 ± 0.0044	0.0039 ± 0.0004	3.95
Nucleotides	Purine	630.8	2.1901 ± 0.3141	1.3194 ± 0.0221	1.66
AA metabolism	Pentanediamine	881.1	0.1703 ± 0.0143	0.0162 ± 0.0011	10.51
AA metabolism	Amine	369.0	0.0734 ± 0.0261	0.0328 ± 0.0036	2.24
Sugar metabolism	Ribitol	784.2	0.6276 ± 0.1768	2.6039 ± 0.1502	0.24
	Glucopyranoside	1561.8	0.1130 ± 0.0198	0.3344 ± 0.0354	0.34
	Gluconolactone-6-P	821.6	0.5679 ± 0.0839	0.7094 ± 0.0181	0.80
	Glycerol	986.0	0.0073 ± 0.0015	0.0103 ± 0.0009	0.71
	Butanediamine	801.1	0.0656 ± 0.0086	0.1224 ± 0.0051	0.54
	Ehylamine	563.0	0.2082 ± 0.0320	0.2436 ± 0.0013	0.85
	Galactose	948.1	1.6122 ± 0.4037	1.9547 ± 0.3306	0.82

*Values represent metabolites abundances (mean ± SD) of 3 replicates from mixed 3 independent samples, as measured by GC-Tof-MS in 3-day mycelia (normalized by C17:0 fatty acid). R.T.: retention time; TCA: tricarboxylic acid; AA: amino acid.

ISSN: 1471-2180