S. epidermidis isolates associated with catheter infection exhibit greater biofilm self-renewal. Laboratory strain ATCC 35984 and clinical isolates Se-1, Se-2, Se-3 and Se-4 were grown for ~7 days in flow chambers irrigated with minimal medium, and stained with SYTO 9 and PI at indicated time points to identify live and dead cells, respectively. Microscopic investigation was performed using confocal laser scanning microscopy (CLSM). The central pictures show horizontal optical sections, and the flanking pictures show side views. Live cells appear green and dead cells appear yellow/red. Bars, 50 μm.