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Figure 4 | BMC Microbiology

Figure 4

From: An important role of interleukin-10 in counteracting excessive immune response in HT-29 cells exposed to Clostridium butyricum

Figure 4

Disruption of IL-10 induces apoptosis and necrosis of HT-29 cells treated with C. butyricum . IL-10 antibody or siIL-10 treated cells were stimulated by C. butyricum. (A) After a 2 h incubation, cells were stained with DAPI and PI. Left: staining with DAPI; middle: PI immunocytochemistry; Right: merge of the two stains. A1, A2 and A3 indicated HT-29 cells of the control, IL-10 antibody and siIL-10 treated groups respectively. (B) Caspase-3 activation was measured using the chromogenic substrate Ac-DEVD-Pna. (C) DNA fragmentation was detected using 1.0% agarose gel electrophoresis. M: 2,000 bp ladder markers. Control: the cells treated with C. butyricum.

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BMC Microbiology

ISSN: 1471-2180

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