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Table 3 Analysis of mangotoxin production using miniTn5 and insertional mutants obtained from Pseudomonas syringae pv.syringae UMAF0158, before and after transformation with plasmids containing total or partial mgo operon involved in mangotoxin production

From: Characterisation of the mgo operon in Pseudomonas syringae pv. syringae UMAF0158 that is required for mangotoxin production

   Mangotoxin production in strains containing complementing plasmidsa
Strain ORF mutated None pCG2-6 pLac36 pLac56 pLac6
UMAF0158 nab + na na na na
UMAF0158::mgoB mgoB + + + + +
UMAF0158-3νH1 mgoC - + + - -
UMAF0158::mgoC mgoC - + + - -
UMAF0158-6νF6 mgoA - + + + -
UMAF0158::mgoA mgoA - + + + -
UMAF0158::mgoD mgoD - + + + +
  1. a) +: presence of inhibition halo; -: absence of inhibition halo
  2. b) na, not applicable
  3. pCG2-6: genomic clone of UMAF0158. GenBank access DQ532441 (Table 4)
  4. pLac36: mgoB, mgoC, mgoA and mgoD cloned in pBBR1MCS-5 (Table 4)
  5. pLac56: mgoA and mgoD cloned in pBBR1MCS-5 (Table 4)
  6. pLac6: mgoD cloned in pBBR1MCS-5 (Table 4)