Table 1 Primer pairs and probes used in this study

For cloning p28-Omp14 and p28-Omp19promoters into pMT504

RRG217 (p28-Omp14)

5"-ttgctcaaccataaaataatggga

F

25

RRG695 (p28-Omp14)

5"-taaaaatttaagaataatgaaag

R

This study

RRG185 (p28-Omp19)*^#

5"-GACTCTAGActtttaattttattattgccacatg

F

25

RRG696 (p28-Omp19)

5"-aaataaattaacaatagtagaag

R

This study

For cloning RpoD gene into pET32

RRG742*

5"-GAGCCATGGcttaacaaattctatattttccctaactc

F

This study

RRG743*

5"-CGCTCGAGttaactattgatattacaatgacctagt

R

This study

For TaqMan RT-PCR of test G-less transcripts

RRG766

5"-ccttcctccatctataccac

F

This study

RRG767

5"-gagagtgaatgatgatagatttg

R

This study

RRG765 (TaqMan Probe)

5"-cattattcctcctatcttctcctcttctc

This study

For TaqMan RT-PCR of control G-less transcripts

RRG769

5"-tactcacccaatactcccta

F

This study

RRG770

5"-gtggaatgagaaatgagtgt

R

This study

RRG768 (TaqMan Probe)

5"-cttatcctctcctcacctctccctc

This study

For sequencing pRG198

M13F-40

5"-gttttcccagtcacgac

Commercial

p28-Omp14 promoter EMSA probes

   Full length probe

RRG 217**

F

RRG 218

5' gttaataaaccttttataaaag

R

25

   Probe 1 (P1)

RRG217**

F

RRG623

5"-ggtttagccattttaaatgtg

R

This study

   Probe 2 (P2)

RRG267

5"-cagttaactttctgtaaacttc

F

25

RRG623

5"-ggtttagccattttaaatgtg

R

This study

   Probe 3 (P3)

RRG269

5"-cgttttctgctttattagaatg

F

25

RRG625

5"-gtacatgcattatgagcaaatc

R

This study

   Probe 4 (P4)

RRG270

5"-gttccgtatttattaatatatg

F

25

RRG626

5"-ctatacttaactttactactta

R

This study

   Probe 5 (P5)

RRG272

5"-ggataagtactttagcaagtgg

F

25

RRG627

5"-gtctagaatataaaatttctttc

R

This study

p28-Omp19 promoter EMSA probes

   Full length probe

RRG 185**

F

25

RRG 445

5' atataacctaatagtgacaaataaattaac

R

This study

   Probe 6 (P6)

RRG185**

F

25

RRG628

5"-gcacttataaactagtccc

R

This study

   Probe 7 (P7)

RRG276

5"-gtgctgtttttctcacctttacac

F

25

RRG629

5"-cttttgtaaggaaaatttaatata

R

This study