Figure 3From: Isolation and characterization of Ehrlichia chaffeensis RNA polymerase and its use in evaluating p28 outer membrane protein gene promotersConstruction of transcription plasmids, pRG147and pRG198. The plasmids were constructed by cloning PCR-amplified E. chaffeensis-specific promoters of p28-Omp14 (pRG147) and p28-Omp19 (pRG198) into the EcoRV located upstream of a G-less cassette in pMT504 [26].Back to article page