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Table 1 Effect of glnB and glnK mutations on nlmAglnKamtB expression

From: Role of PII proteins in nitrogen fixation control of Herbaspirillum seropedicae strain SmR1

Growth Conditions

β-galactosidase Activity [nmol o-nitrophenol/(min.mg protein)]

 

Strains

 

LNamtBlacZ

(SmR1, amtB::lacZ )

LNglnKamtBlacZ

(Δ glnK , amtB::lacZ )

LNglnBamtBlacZ

( glnB -TcR, amtB::lacZ )

5 mmol/L glutamate

(2.5 ± 0.2) × 103

(2.4 ± 0.2) × 103

(2.3 ± 0.2) × 103

2 mmol/L NH4Cl

(2.1 ± 0.1) × 103

(2.29 ± 0.08) × 103

(2.2 ± 0.1) × 103

20 mmol/L NH4Cl

(1.1 ± 0.2) × 102

(1.4 ± 0.4) × 102

(1.6 ± 0.3) × 102

  1. Indicated strains of H. seropedicae were grown in the presence of glutamate or NH4Cl. β-galactosidase activity was determined as described. Values are the mean of at least three independent experiments ± standard deviation.