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Table 1 Effect of glnB and glnK mutations on nlmAglnKamtB expression

From: Role of PII proteins in nitrogen fixation control of Herbaspirillum seropedicae strain SmR1

Growth Conditions β-galactosidase Activity [nmol o-nitrophenol/(min.mg protein)]
  Strains
  LNamtBlacZ
(SmR1, amtB::lacZ )
LNglnKamtBlacZ
glnK , amtB::lacZ )
LNglnBamtBlacZ
( glnB -TcR, amtB::lacZ )
5 mmol/L glutamate (2.5 ± 0.2) × 103 (2.4 ± 0.2) × 103 (2.3 ± 0.2) × 103
2 mmol/L NH4Cl (2.1 ± 0.1) × 103 (2.29 ± 0.08) × 103 (2.2 ± 0.1) × 103
20 mmol/L NH4Cl (1.1 ± 0.2) × 102 (1.4 ± 0.4) × 102 (1.6 ± 0.3) × 102
  1. Indicated strains of H. seropedicae were grown in the presence of glutamate or NH4Cl. β-galactosidase activity was determined as described. Values are the mean of at least three independent experiments ± standard deviation.