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Table 1 Purification of mutacins F-59.1 and D-123.1 by hydrophobic chromatography.

From: Production, purification, sequencing and activity spectra of mutacins D-123.1 and F-59.1

Step Volume
(mL)
Activity
(AU/mL)
Total Protein
(mg)
Total activity
(AU.103)
Specific activity
(AU/mg)
Yield
(%)
Purification
(fold)
mutacin F-59.1        
Culture supernatant 1000 400 10000 400 40 100 1
Sep-Pak C18 95 3200 3000 304 101 76 2.5
C18 RP-HPLC 2 16000 0.1 32 3.2 × 105 8 8 × 103
mutacin D-123.1        
Culture supernatant 675 200 4320 135 31 100 1
Sep-Pak C18 50 1600 8 80 1 × 104 59 320
C18 RP-HPLC 1 800 0.005 0.8 1.6 × 105 0.2 5120