Skip to main content

Advertisement

Table 1 Bacterial strains and plasmid.

From: Housekeeping genes essential for pantothenate biosynthesis are plasmid-encoded in Rhizobium etli and Rhizobium leguminosarum

Strain or plasmid Relevant genotype Reference or source
Rhizobium etli   
CFN42 Wild type; Nalr [6]
ReTV1 CFN42 panC::pTV1; Kmr This study
ReTV1-4 CFN42 panC::pTV1 complemented with pTV4; Tcr Kmr This study
ReTV1-5 CFN42 panC::pTV1 complemented with pTV5; Tcr Kmr This study
ReTV2 CFN42 panB::pTV2; Kmr This study
ReTV2 -4 CFN42 panB::pTV2 complemented with pTV4; Tcr Kmr This study
ReTV2 -6 CFN42 panB::pTV2 complemented with pTV6; Tcr Kmr This study
ReTV2 -7 CFN42 panB::pTV2 complemented with PTV7; Tcr Kmr This study
ReTV3 CFN42 argE::pTV3; Kmr This study
CFNX186 CFN42 cured of plasmid p42f; Nalr [18]
CFNX186-4 CFNX186 complemented with pTV4; Tcr This study
CFNX186-24 CFNX186 complemented with pCos24; Tcr [30]
CIAT 652 Wild type; Nalr [38]
CIAT 894 Wild type; Nalr [38]
Kim5 Wild type; Nalr J. Handelsman, University of Wisconsin, MD
IE4771 Wild type; Nalr [15]
Escherichia coli   
DH5α Host for recombinant plasmids; Nalr Stratagene
S17-1 C600::RP4-2(Tc::Mu) (Km::Tn7) Donor for conjugation [39]
Plasmids   
pBC pBluescript II SK(+) phagemid vector; Cmr Stratagene.
pK18mob pK18, derivative mob; Kmr [29]
pRK7813 Broad-host-range cosmid vector; Mob, IncP, Tcr [40]
pBBRMCS3 Broad-host-range cloning vector; Mob; Tcr [41]
pBC1 pBC harboring a 400-bp BamHI-XbaI PCR fragment of panC; Cmr This study
pBC2 pBC harboring a 400-bp BamHI-XbaI PCR fragment of panB; Cmr This study
pTV1 pK18mob harboring a 400-bp KpnI-XbaI PCR fragment of panC; Kmr This study
pTV2 pK18mob harboring a 400-bp KpnI-XbaI PCR fragment of panB; Kmr This study
pTV3 pK18mob harboring a 400-bp KpnI-XbaI PCR fragment of argE; Kmr This study
pTV4 pRK7813 harboring a 3.1 kb EcoRI fragment of pCos24 containing panC and panB; Tcr This study
pTV5 pBBRMCS3 harboring a 1.2 kb KpnI-XbaI PCR fragment containing panC; Tcr This study
pTV6 pBBBRMCS3 harboring a 1 kb KpnI-XbaI PCR fragment containing panB; Tcr This study
pTV7 pBBRMCS53 harboring a 1 kb KpnI-XbaI PCR fragment containing RHE_PE00443; Tcr This study
pcos24 20 Kb EcoRI fragment of plasmid p42f cloned in pLAFR1 containing panC, panB, oxyR and katG; Tcr [30]