Table 1 Bacterial strains and plasmid.

Rhizobium etli

CFN42

Wild type; Nal^r

[6]

ReTV1

CFN42 panC::pTV1; Km^r

This study

ReTV1-4

CFN42 panC::pTV1 complemented with pTV4; Tcr Km^r

This study

ReTV1-5

CFN42 panC::pTV1 complemented with pTV5; Tcr Km^r

This study

ReTV2

CFN42 panB::pTV2; Km^r

This study

ReTV2 -4

CFN42 panB::pTV2 complemented with pTV4; Tc^r Km^r

This study

ReTV2 -6

CFN42 panB::pTV2 complemented with pTV6; Tc^r Km^r

This study

ReTV2 -7

CFN42 panB::pTV2 complemented with PTV7; Tc^r Km^r

This study

ReTV3

CFN42 argE::pTV3; Km^r

This study

CFNX186

CFN42 cured of plasmid p42f; Nal^r

[18]

CFNX186-4

CFNX186 complemented with pTV4; Tc^r

This study

CFNX186-24

CFNX186 complemented with pCos24; Tc^r

[30]

CIAT 652

Wild type; Nal^r

[38]

CIAT 894

Wild type; Nal^r

[38]

Kim5

Wild type; Nal^r

J. Handelsman, University of Wisconsin, MD

IE4771

Wild type; Nal^r

[15]

Escherichia coli

DH5α

Host for recombinant plasmids; Nal^r

Stratagene

S17-1

C600::RP4-2(Tc::Mu) (Km::Tn7)

Donor for conjugation

[39]

Plasmids

pBC

pBluescript II SK(+) phagemid vector; Cm^r

Stratagene.

pK18mob

pK18, derivative mob; Km^r

[29]

pRK7813

Broad-host-range cosmid vector; Mob, IncP, Tc^r

[40]

pBBRMCS3

Broad-host-range cloning vector; Mob; Tc^r

[41]

pBC1

pBC harboring a 400-bp BamHI-XbaI PCR fragment of panC; Cm^r

This study

pBC2

pBC harboring a 400-bp BamHI-XbaI PCR fragment of panB; Cm^r

This study

pTV1

pK18mob harboring a 400-bp KpnI-XbaI PCR fragment of panC; Km^r

This study

pTV2

pK18mob harboring a 400-bp KpnI-XbaI PCR fragment of panB; Km^r

This study

pTV3

pK18mob harboring a 400-bp KpnI-XbaI PCR fragment of argE; Km^r

This study

pTV4

pRK7813 harboring a 3.1 kb EcoRI fragment of pCos24 containing panC and panB; Tc^r

This study

pTV5

pBBRMCS3 harboring a 1.2 kb KpnI-XbaI PCR fragment containing panC; Tc^r

This study

pTV6

pBBBRMCS3 harboring a 1 kb KpnI-XbaI PCR fragment containing panB; Tc^r

This study

pTV7

pBBRMCS53 harboring a 1 kb KpnI-XbaI PCR fragment containing RHE_PE00443; Tc^r

This study

pcos24

20 Kb EcoRI fragment of plasmid p42f cloned in pLAFR1 containing panC, panB, oxyR and katG; Tc^r

[30]