Lateral gene transfer of streptococcal ICE element RD2 (region of difference 2) encoding secreted proteins

BMC Microbiology

Table 2 Detection of RD2 element genes in Lancefield group C and G streptococci by PCR.

A. Detection of genes encoding putative extracellular proteins
Strain	M28_ Spy1306	M28_ Spy1307		M28_ Spy1308		M28_ Spy1325		M28_ Spy1326		M28_ Spy1332		M28_ Spy1336
GCS
15169	+	+		-		+		+		-		+
15170	+	+		-		-		+		-		-
15172	+	+		+		+		+		-		+
15173	+	+		-		+		+		-		+
15178	+	+		+		+		+		+		+
15181	+	+		-		+		+		-		+
GGS
15163	+	+		-		+		+		-		+
15164	+	+		-		+		+		-		+
15165	+	+		-		+		+		-		+
15166	+	+		-		+		+		-		+
15167	+	+		-		+		+		-		+
15168	+	+		-		+		+		-		+
15171	+	+		+		+		+		-		+
15174	+	+		+		+		+		+		+
15175	+	+		+		-		-		-		-
15176	+	+		-		+		+		-		+
15177	+	+		-		+		+		-		+
15179	+	+		-		+		+		-		+
15180	+	+		+		+		+		-		+
15182	+	+		+		+		+		+		+
B. PCR-tiling across the entire RD2 element. Example of the tiling across RD2 is presented in Figure 3. (+) PCR product present, (-) no product, * amplified fragment of different size than for strain MGAS6180
		PCR-tiling fragment no.
Strain	group	1	2	3	4	5	6	7	8	9	10	11	12
6180	A	+	+	+	+	+	+	+	+	+	+	+	+
15178	C	-	+	+	+	+	-	-	+	-	-	+	-
15174	G	+(*)	+	+	+	+	+	-	+	+	+	+	-
15182	G	-	+	+	+	+	+	+	+	+	+	+	-

ISSN: 1471-2180