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Table 2 Detection of RD2 element genes in Lancefield group C and G streptococci by PCR.

From: Lateral gene transfer of streptococcal ICE element RD2 (region of difference 2) encoding secreted proteins

A. Detection of genes encoding putative extracellular proteins
Strain M28_ Spy1306 M28_ Spy1307 M28_ Spy1308 M28_ Spy1325 M28_ Spy1326 M28_ Spy1332 M28_ Spy1336
GCS
15169 + + - + + - +
15170 + + - - + - -
15172 + + + + + - +
15173 + + - + + - +
15178 + + + + + + +
15181 + + - + + - +
GGS
15163 + + - + + - +
15164 + + - + + - +
15165 + + - + + - +
15166 + + - + + - +
15167 + + - + + - +
15168 + + - + + - +
15171 + + + + + - +
15174 + + + + + + +
15175 + + + - - - -
15176 + + - + + - +
15177 + + - + + - +
15179 + + - + + - +
15180 + + + + + - +
15182 + + + + + + +
B. PCR-tiling across the entire RD2 element. Example of the tiling across RD2 is presented in Figure 3. (+) PCR product present, (-) no product, * amplified fragment of different size than for strain MGAS6180
   PCR-tiling fragment no.
Strain group 1 2 3 4 5 6 7 8 9 10 11 12
6180 A + + + + + + + + + + + +
15178 C - + + + + - - + - - + -
15174 G +(*) + + + + + - + + + + -
15182 G - + + + + + + + + + + -