Bacillus anthracis spore interactions with mammalian cells: Relationship between germination state and the outcome of in vitro

Table 1 Germination and outgrowth of B. anthracis spores as a function of cell culture medium in the presence or absence of FBS^a.

^a Three independent experiments were performed with three different spore preparations, each conducted in triplicate.
^b Spores prepared from B. anthracis Sterne 7702 were incubated in the indicated medium.
^c Indicates the presence (+) or absence (-) of 10% FBS in the indicated medium.
^d Spores were scored positive (+) for germination if the OD_{600 nm} of the suspended spores decreased by more than 10% after 30 min incubation in the indicated medium.
^e Using DIC microscopy, spores were scored positive (+) for outgrowth if the spores bodies were visibly larger at 1 h, and had developed into vegetative bacteria by 4 h.
^f AA refers to L-alanine and L-inosine (each at 10 mM, in PBS pH 7.2).

ISSN: 1471-2180