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Table 1 Germination and outgrowth of B. anthracis spores as a function of cell culture medium in the presence or absence of FBSa.

From: Bacillus anthracis spore interactions with mammalian cells: Relationship between germination state and the outcome of in vitro

    outgrowthe
mediumb FBSc germinationd 1 h 4 h
DMEM - - - -
  + + + +
RPMI - - - -
  + + + +
MEMα - + + +
  + + + +
MEM - - - -
  + + + +
AMEM - - - -
  + + + +
EMEM - - - -
  + + + +
BME - - - -
  + + + +
CIM - + + +
  + + + +
F-12 - - - -
  + + + +
M5A - + + +
  + + + +
BHI - + + +
LB - + + +
AAf - + - -
  1. a Three independent experiments were performed with three different spore preparations, each conducted in triplicate.
  2. b Spores prepared from B. anthracis Sterne 7702 were incubated in the indicated medium.
  3. c Indicates the presence (+) or absence (-) of 10% FBS in the indicated medium.
  4. d Spores were scored positive (+) for germination if the OD600 nm of the suspended spores decreased by more than 10% after 30 min incubation in the indicated medium.
  5. e Using DIC microscopy, spores were scored positive (+) for outgrowth if the spores bodies were visibly larger at 1 h, and had developed into vegetative bacteria by 4 h.
  6. f AA refers to L-alanine and L-inosine (each at 10 mM, in PBS pH 7.2).