Figure 2

Autoradiogram of SDS-PAGE-separated M. tuberculosis His ₁₀ -Obg after autophosphorylation. Autophosphorylation reactions were set up by incubating 5 μg of His₁₀-Obg with 10 μCi of [γ-³²P] GTP in autophosphorylation buffer, as detailed in the Methods section. A. Autophosphorylation of His₁₀-Obg by [γ-³²P] GTP or [γ-³²P]ATP after 0, 15, 30 and 60 minutes of incubation at 37°C. B. Autophosphorylation of His₁₀-Obg by [γ-³²P]GTP in the presence (+ lane) and absence of (- lane) 1.5 mM MgCl₂ . C. Autophosphorylation of His₁₀-Obg by [γ-³²P]GTP in the presence of 5 mM (Lane 1), 50 mM (Lane 2) and 500 mM (Lane 3) ATP; 5 mM (Lane 1), 50 mM (Lane 2) and 500 mM (Lane 3) of GTP; 5 mM (Lane 1), 50 mM (Lane 2) and 500 mM (Lane 3) of GDP.