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Table 2 Plasmids

From: Role of ZnuABC and ZinT in Escherichia coliO157:H7 zinc acquisition and interaction with epithelial cells

Plasmid Relevant genotype or characteristic Reference or source
pKD46 lambda red recombinase function Datsenko and Wanner, 2000
pKD3 chloramphenicol resistance cassette template Datsenko and Wanner, 2000
pKD4 kanamycin resistance cassette template Datsenko and Wanner, 2000
pSUB11 3xFLAG-kanamycin resistance cassette template Uzzau et al., 2001
p18ZnuAO157 ZnuA of E. coli O157:H7 cloned in pEMBL18 This work
p18ZnuAE. coli ZnuA of E. coli K12 cloned in pEMBL18 This work