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Table 2 Effect of efflux inhibitors on the MICs of antibiotics for wild-type and mutant strains of M. smegmatis

From: Ethidium bromide transport across Mycobacterium smegmatiscell-wall: correlation with antibiotic resistance

MICs (mg/L)

  

M. smegmatisstrains

Antibiotic/EPI

mc2155 (wild-type)

SMR5 (mc2155 STRr)

MN01 (SMR5 Δ mspA )

ML10 (SMR5 Δ mspA Δ mspC )

XZL1675 (mc2155 Δ lfrA )

XZL1720 (mc2155 Δ lfrR )

 

No EPI

0.5

0.5

0.5

0.5

0.5

0.5

AMK

CPZ

0.125

0.125

0.125

0.25

0.063

0.063

 

TZ

0.063

0.063

0.125

0.25

0.063

0.063

 

VP

0.125

0.125

0.125

0.25

0.125

0.125

 

No EPI

0.25

0.25

0.25

0.25

0.125

0.125

CIP

CPZ

0.063

0.063

0.063

0.063

0.063

0.063

 

TZ

0.063

0.063

0.063

0.063

0.032

0.032

 

VP

0.063

0.063

0.063

0.063

0.063

0.063

 

No EPI

2

2

8

8

2

2

CLT

CPZ

0.25

0.25

0.5

1

0.25

0.25

 

TZ

0.25

0.25

1

1

0.25

0.25

 

VP

0.5

0.5

0.5

1

0.5

0.5

 

No EPI

1

1

1

1

0.5

1

EMB

CPZ

1

1

1

1

0.5

1

 

TZ

1

1

1

1

0.5

1

 

VP

1

1

1

1

0.5

1

 

No EPI

32

32

64

64

32

32

ERY

CPZ

4

4

8

8

4

4

 

TZ

4

4

16

16

4

4

 

VP

8

8

8

8

8

8

 

No EPI

4

4

8

8

0.5

0.5

RIF

CPZ

1

1

2

2

0.125

0.125

 

TZ

2

2

4

4

0.125

0.125

 

VP

2

2

4

4

0.125

0.25

 

No EPI

0.5

>256

>256

>256

0.5

0.5

STR

CPZ

0.125

>256

>256

>256

0.032

0.063

 

TZ

0.125

>256

>256

>256

0.125

0.25

 

VP

0.25

>256

>256

>256

0.25

0.125

  1. AMK, amikacin; CIP, ciprofloxacin; CLT, clarithromycin; CPZ, chlorpromazine; EMB, ethambutol; EPI, efflux pump inhibitor; ERY, erythromycin; RIF, rifampicin; STR, streptomycin; TZ, thioridazine; VP, verapamil. Data in bold type represents significant (at least 4-fold) reduction of the MIC produced by the presence of an efflux inhibitor.
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BMC Microbiology

ISSN: 1471-2180

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