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Figure 1 | BMC Microbiology

Figure 1

From: Studies on Escherichia coliHflKC suggest the presence of an unidentified λ factor that influences the lysis-lysogeny switch

Figure 1

Role of HflKC on in vivo proteolysis of CII. Left panel shows the proteolytic pattern of exogenous CII (expressed from pKP219) in wild type cells (open circles), AK990 (ΔhflKC, squares) or wild type cells carrying plasmid pQKC (triangles). In each case, the expression of CII was induced with IPTG and translation was stopped 30 minutes later with spectinomycin. Relative amount of CII was measured after regular intervals (0, 5, 10, 15, 20 minutes) by western blotting followed by quantification using densitometric analysis. Corresponding western blots showing the stability of CII in different host strains are shown in the right panel.

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BMC Microbiology

ISSN: 1471-2180

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