Table 5 Oligonucleotide primers used in this study
From: Repression of btuB gene transcription in Escherichia coliby the GadX protein
Primer | Sequence 5 '- 3' |
|---|---|
F/cea7-BamHI | GGATCCATGAGCGGTGGAGATGGACG |
R/cei7-PstI | CTGCAGTCAGCCCTGTTTAAATCC |
F/btuB-219-XbaI | GGCTCTAGAAAACGGTGCCATCATACTTTG |
R/btuB+242-HindIII | GGCAAGCTTATCATTGTAAAGCATCCACAATAG |
F/btuB-767 | GTTCACCGTTGCTCGATACC |
R/btuB-1087 | TCAGATAGATGCCGGTATTACG |
F/btuB-431-XbaI | GCTCTAGAACGGGATTATTACGC |
F/btuB-671-XbaI | GCTCTAGATCATCTCTTTCCC |
F/btuB-1043-XbaI | GCTCTAGACCGCTGCGCGGA |
R/lacZ | TTATTTTTGACACCAGACC |
F/gadA-176 | GATCGCCCGAACAGCAA |
R/gadA+77 | CGTGAATCGAGTAGTTC |
F/gadB-173 | AATAACAGCATAAAACA |
R/gadB+77 | CGTGAATCGAGTAGTTCC |
F/pal-XbaI | TCTAGAGAGGCGTACAAGTTCTG |
R/pal-HindIII | AAGCTTATCATTTCAATGATTCCTTTAC |
F/gadX-BamHI | GGATCCATGCAACCACTACATGG |
R/gadX-PstI | CTGCAGCTATAATCTTATTCCTT |
F/gadX-393 | TATACCGCTGCTTCTGAACG |
R/gadX-726 | TCGCTCCTGATACTCTGTGG |
F/rrsA-483 | CGTTACCCGCAGAAGAAGC |
R/rrsA-808 | GTGGACTACCAGGGTATCTAATCC |