Improving Salmonella vector with recmutation to stabilize the DNA cargoes

BMC Microbiology

Table 3 Plasmid recombination frequency (Mean ± STD, × 10^-³)

Strain	recdeletion	pYA4463^a	pYA4590^b	pYA4464+pYA4465^c
S. Typhimurium
χ3761	None	1.55 ± 0.31	2.40 ± 0.54	2.88 ± 0.85
χ9833	ΔrecA62	1.07 ± 0.24	0.22 ± 0.07**	0.27 ± 0.07**
χ9070	ΔrecF126	1.14 ± 0.15	0.52 ± 0.07**	0.33 ± 0.09**
χ9072	ΔrecJ1315	1.87 ± 0.44	2.37 ± 0.21	1.10 ± 0.20**
χ9081	ΔrecJ1315 ΔrecF126	NA^d	NA	0.35 ± 0.08**
χ9939	ΔrecF126 ΔrecA62	NA	0.41 ± 0.09**	0.35 ± 0.08**
χ9833(pYA5002)	ΔrecA62 (RecA⁺)	NA	2.50 ± 0.42	NA
χ9070(pYA5005)	ΔrecF126 (RecF⁺)	NA	2.00 ± 0.24	NA
S. Typhi Ty2
χ3769	None	4.69 ± 0.26	11.59 ± 2.61	4.20 ± 1.44
χ11159	ΔrecA62	1.32 ± 0.27**	0.60 ± 0.19**	3.37 ± 0.96
χ11053	ΔrecF126	0.51 ± 0.06**	0.57 ± 0.09**	6.19 ± 2.71
χ11134	ΔrecF1074	0.45 ± 0.05**	0.52 ± 0.17**	16.28 ± 2.64**
χ11194	ΔrecJ1315	1.69 ± 0.26**	4.88 ± 1.56**	2.31 ± 0.90
χ11053(pYA5005)	ΔrecF126 (RecF⁺)	2.52 ± 0.18	NA	NA
χ11053(pYA5006)	ΔrecF126 (RecF⁺)	1.71 ± 0.68	NA	NA
χ11159(pYA5002)	ΔrecA62 (RecA⁺)	NA	14.35 ± 2.44	NA
χ11053(pYA5006)	ΔrecF126 (RecF⁺)	NA	2.86 ± 0.59	NA
S. Typhi ISP1820
χ3744	None	4.93 ± 0.67	13.10 ± 1.23	4.22 ± 0.25
χ11133	ΔrecF1074	0.65 ± 0.26**	0.71 ± 0.06**	5.38 ± 0.58
S. Paratyphi A
χ8387	None	2.70 ± 0.39	3.32 ± 0.61	1.03 ± 0.15
χ11243	ΔrecA62	1.91 ± 0.69**	0.55 ± 0.20**	0.13 ± 0.03**
χ11244	ΔrecF126	5.00 ± 0.70	1.16 ± 0.21**	0.34 ± 0.04**
χ11245	ΔrecJ1315	2.56 ± 0.41	1.83 ± 0.99**	0.64 ± 0.15**

^aIntraplasmid recombination without intervening sequence (5'tet-3'tet).
^b Intraplasmid recombination with a 1041-bp intervening sequence (5'tet-kan-3'tet).
^c Interplasmid recombination.
^d Not assayed.
** P < 0.01, relative to the parental rec⁺ strain.

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