Table 2 Primers used in this study.
Target gene | Primer sequences (5' → 3') | Amplicon size (bp) | Application |
|---|---|---|---|
ef1415 | F:TGTTGCGGTTTCTGCATTAG | 2818 | PCR on junction between EF1415 and EF1417 |
ef1417 | R:GCATCTCGATAGACAATTCG | Â | PCR on junction between EF1415 and EF1417 |
ef1489 | F:GAATCGAACTAGCATTTTTGGG | 465 | PCR on junction between EF1489 and EF1490 |
ef1490 | R:ATGGAACGAACCATTGGAAA | Â | PCR on junction between EF1489 and EF1490 |
ef1843 | F:GGAGCCGTTAGACAGACAGC | 2457 | PCR on junction between EF1843 and EF1847 |
ef1847 | R:GCTTGCTTTACAGCCTCAAGA | Â | PCR on junction between EF1843 and EF1847 |
ef1895 | F:GCACAACAAATTTCAATTCCA | 4573 | PCR on junction between EF1895 and EF1898 |
ef1898 | R:ATTGAAGTGGTTCGCTACGG | Â | PCR on junction between EF1895 and EF1898 |
ef2239 | F:AACTGCTGTCAAGCGTAGCA | 1252 | PCR on junction between EF2239 and EF2240 |
ef2240 | R:TGTGGCATTTTGGACTGTTG | Â | PCR on junction between EF2239 and EF2240 |
ef2350 | F:ATAACTGAGTGATTTTCACAATTGC | 654 | PCR on junction between EF2350 and EF2352 |
ef2352 | R:GATCCGTGGAAGTTCCTCAA | Â | PCR on junction between EF2350 and EF2352 |
ef3216 | F:TCGGCGTTGAAGACTATGAA | - | Sequencing of junction between EF3216 and EF3230 |
ef3217 | F:ATTGGGAATGACGGCTACAC R:TTGCGTATTTCGCAGCATAA | 499 | PCR |
ef3218 | F:TCGCGTAGTAGGAGCAATCA R:TTTTGTTCAGTTCCCACACCT | 396 | PCR |
ef3220 | F:AGCTTTTGGCGAAGGAGATT R:TTTATTGCGGGTTCCTCAGT | 495 | PCR |
ef3221 | F:TGAACGAAAATGAAGGTGGT R:TCATCAATCTCCAACGCATC | 196 | PCR |
ef3222 | F:CAAAGAAGAATCAGCCGATTAAA R:ATATTTGGGCATTTGCATGG | 183 | PCR |
ef3223 | F:AATTGGGAAAAAGGGGTCAG R:TTCGTGATCTGCTTGTTGTTCT | 501 | PCR |
ef3224 | F:GTTGGGCTGGACGTATGAAT R:TGTGGCTTTATAGGCTGTAGCA | 214 | PCR |
ef3225 | F:ATTACTTCACCGCCCATGAC R:CGCTGGAAGTCTGCTCTTG | 474 | PCR |
ef3226 | F:GATGATTTAACCGCACAAGGA R:TTTTTATTTCGAGCGGATGC | 499 | PCR |
ef3227 | F:ACAGGAAGCCATTCACAAACT R:CTGATTCGTGGAAGTCCAACT | 162 | PCR |
ef3230 | R:TCCTGACTTCCGTTCTGCTT | - | Sequencing of junction between EF3216 and EF3230 |
hmpref0346_1861 | F:CGAGTTAGAGGAAGCGTTGG | 630 | PCR |
| Â | R:CCAGACAATTTGGGCGTACT | Â | Â |
hmpref0346_1864 | F:GAAATTTTCTGAAAGTGAAGACAAGA | 299 | PCR |
| Â | R:TGATTAGCAGTCACAACAGCAA | Â | Â |
hmpref0346_1868 | F:TGTACACAAGCTACCCGGATT | 538 | PCR |
| Â | R:TTCCCACCTGCGTCTATTTT | Â | Â |
hmpref0348_0427 | R:GAGACTTCAACCACTCCACAAAAACC | - | Sequencing of gap between contig00034-35 in TX0104 |
hmpref0348_0428 | F:CCTGTAGAAGTATTGTCCATTTTAACGCTATC | Â | Sequencing of gap between contig00034-35 in TX0104 |