Partial dendrogram MLVA-16 clustering analysis of 170 Brucella isolates, including the 48 isolates from Brucella species that were not B. melitensis or B. abortus included in this study. The columns present data as described in Figure 1. The similarity axis is presented in the top left corner. Each color reflects a different cluster with > 52.5% similarity. The group of 'non-melitensis/abortus' isolates clustered as follows: Cluster 8 with B. suis biovar 3 and B. canis; Cluster 9 with B. suis biovar 1; Cluster 10 with B. suis biovar 2; and Cluster 11 with B. ovis isolates. The 'B. ceti/pinnipedialis/neotomae' subgroup clustered as follows: Clusters 12 and 13 with B. pinnipedialis isolates and Cluster 14 and 16 with B. ceti isolates. Furthermore, this subgroup also contained two clusters with only one isolate (singletons): Cluster 15 with a B. suis biovar 5 and Cluster 16 with a B. neotomae isolate.