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Table 4 E. coli strains and plasmids used in this study

From: Resistance to topoisomerase cleavage complex induced lethality in Escherichia coli via titration of transcription regulators PurR and FNR

  Relevant genotype Source or construction
E. coli strains
BW27784 Δ(araBAD)567 Δ(rhaBAD)568 Yale E. coli Genetic Stock Center
  Δ(araFGH) Φ(ΔaraEpPCP18-araE) [32]
BW117N BW27784 with chromosomally integrated YpTOP1-D117N gene [10]
AQ4335 Δara leu7697 NBRP NBRP-E. coli at NIG
FB20344 MG1655 ydeA::Tn5KAN-I-SceI U. Wisconsin [34]
YT103 AQ4335 ydeA::Tn5KAN-I-SceI P1(FB20344) × AQ4335, Kanr
JW1328-1 Δfnr771::kan Yale E. coli Genetic Stock Center [35]
JW1650-1 ΔpurR746::kan Yale E. coli Genetic Stock Center [35]
IFL6 BW27784 Δ fnr771::kan P1(JW1328-1) × BW27784, Kanr
IFL7 BW27784 Δ purR746::kan P1(JW1650-1) × BW27784, Kanr
Plasmids
pAYTOP128 Mutant derivative of pAYTOP encoding YpTOP1 with G122S, M326V and A383P mutations [11]
pCRII High copy number cloning vector Invitrogen
pAQ5 pCR-XL-TOPO cloning product of E. coli chromosome fragment 2618398-2620765 This study
pAQ5-1 pCR-XL-TOPO carrying upp gene and the intergenic region of upp-purMN This study
pAQ5-2 pCR-XL-TOPO carrying purM gene and the intergenic region of upp-purMN This study
pInter pCR-XL-TOPO carrying the intergenic region of upp-purMN This study
pInterD1 pInter with the FNR binding site deleted This study
pInterD2 pInter with the PurR binding site deleted This study