|
E. coli strains
|
|
BW27784
|
Δ(araBAD)567 Δ(rhaBAD)568
|
Yale E. coli Genetic Stock Center
|
| |
Δ(araFGH) Φ(ΔaraEpPCP18-araE)
|
[32]
|
|
BW117N
|
BW27784 with chromosomally integrated YpTOP1-D117N gene
|
[10]
|
|
AQ4335
|
Δara leu7697 NBRP
|
NBRP-E. coli at NIG
|
|
FB20344
|
MG1655 ydeA::Tn5KAN-I-SceI
|
U. Wisconsin [34]
|
|
YT103
|
AQ4335 ydeA::Tn5KAN-I-SceI
|
P1(FB20344) × AQ4335, Kanr
|
|
JW1328-1
|
Δfnr771::kan
|
Yale E. coli Genetic Stock Center [35]
|
|
JW1650-1
|
ΔpurR746::kan
|
Yale E. coli Genetic Stock Center [35]
|
|
IFL6
|
BW27784 Δ fnr771::kan
|
P1(JW1328-1) × BW27784, Kanr
|
|
IFL7
|
BW27784 Δ purR746::kan
|
P1(JW1650-1) × BW27784, Kanr
|
|
Plasmids
|
|
pAYTOP128
|
Mutant derivative of pAYTOP encoding YpTOP1 with G122S, M326V and A383P mutations
|
[11]
|
|
pCRII
|
High copy number cloning vector
|
Invitrogen
|
|
pAQ5
|
pCR-XL-TOPO cloning product of E. coli chromosome fragment 2618398-2620765
|
This study
|
|
pAQ5-1
|
pCR-XL-TOPO carrying upp gene and the intergenic region of upp-purMN
|
This study
|
|
pAQ5-2
|
pCR-XL-TOPO carrying purM gene and the intergenic region of upp-purMN
|
This study
|
|
pInter
|
pCR-XL-TOPO carrying the intergenic region of upp-purMN
|
This study
|
|
pInterD1
|
pInter with the FNR binding site deleted
|
This study
|
|
pInterD2
|
pInter with the PurR binding site deleted
|
This study
|
