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Table 1 Comparison of culture and qPCR for quantification of Legionella.

From: Detection of Legionella by quantitative-polymerase chain reaction (qPCR) for monitoring and risk assessment

     Number of positive samples Concentrations
     Culture qPCR Culture qPCR
Sampling Sampling site Type of sample No of samples Legionella spp Legionella spp Legionella pneumophila Legionella spp 104 CFU/L Legionella spp104 GU/L Legionella pneumophila 104 GU/L
  Circulation water B 1 1 1 1 5.5 3.4 3.6
Before the first intervention Empty apartment A 0       
  Shower hose A 1 1 1 1 60 26 14
  Circulation water B 10 10 10 10 0.005 - 1.2 [0.08] 0.77 - 2.9 [1.5] 0.6 - 2.6 [1.1]
After the first intervention Empty apartment A 4 4 4 4 1.9 - 33 [19] 2.9 - 24 [8.9] 4.9 - 19 [11]
  Shower hose A 5 5 5 5 0.8 - 160 [27] 3.5 - 96 [28] 1.1 - 43 [17]
  Circulation water B 16 0 16 13 BD 0.4-1.9 [0.62] BD - 2.0 [0.27]
After the second intervention Empty apartment A 2 1 2 2 BD - 0.001 3.2 - 55 [29] 3.7 - 68 [36]
  Shower hose A 8 0 8 8 BD 0.17 - 2.3 [0.95] 0.033 - 3.2 [1.3]
  1. Number of samples and amount of Legionella detected in samples from circulation water, from first flush of taps in empty apartments and from first flush of shower hoses by culture and by Legionella pneumophila and Legionella species qPCR assays before and after interventions. Samples were collected as first flush (A) or after reaching constant temperature (B). BD: Below detection. Median value is given in [..]