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Table 2 Primers used in this study

From: Integration of a laterally acquired gene into a cell network important for growth in a strain of Vibrio rotiferianus

Primer Sequence (5'-3') Target
PRG1-F GTC GAC CAA AAT TTG GCT GCT TGT TG Paralog 1 gene cassettes in Vibrio rotiferianus DAT722
PRG1-R CAT CAG AGA TTT TGA GAC ACA ACC CGA GCG ACA ATT TTA AGC Paralog 1 gene cassettes in Vibrio rotiferianus DAT722
PRG5-F GGC AGA GCA TTA CGC TGA TCA AAG GTC ATA AGT TTT GGT G Paralog 2 gene cassettes in Vibrio rotiferianus DAT722
PRG5-R GTC GAC CAT GCG CTA CTT CTA TTT ATG C Paralog 2 gene cassettes in Vibrio rotiferianus DAT722
Kan-F GTT GTG TCT CAA AAT CTC TGA TG aphA1 in pLOW2 (F)
Kan-R TCA GCG TAA TGC TCT GCC aphA1 in pLOW2 (R)
VSD5-F TGA GCT ACC ACA AGC AAG G Cassette 5 in Vibrio rotiferianus DAT722 (F)
VSD14-F AAA GCG GTT ACA TTC GGG Cassette 5 in Vibrio rotiferianus DAT722 (R)
VSD25a-F ACA TAT GTA GAC CCT GTG CG Cassette 25 in Vibrio rotiferianus DAT722 (F)
VSD47-F CAT TTT AAG TCG GCT CTT CC Cassette 25 in Vibrio rotiferianus DAT722 (R)
VSD62-R GTA GGT AAT TTC GGC TTC TCG Cassette 62 in Vibrio rotiferianus DAT722 (R)
VSD25b-F TGC GCA ATA TAT CGC AAG AG Cassette 25 in Vibrio rotiferianus DAT722 (F)
VSD25-R GCC GTC CAT AGT CGT CAT TT Cassette 25 in Vibrio rotiferianus sp. DAT722 (R)
B-VSD11-F TTT TGG ATC CGA ATA GGG AAA ATC CGT G Gene from cassette 11 in V. rotiferianus DAT722 (F)
P-VSD11-R TTT TCT GCA GTT AGT TGA ATT GTT TCA CAG C Gene from cassette 11 in V. rotiferianus DAT722 (R)