Figure 2From: The uncertain consequences of transferring bacterial strains between laboratories - rpoSinstability as an exampleAccumulation of low-RpoS mutants in LB-stabs. Ten LB-stabs were inoculated with a single colony of MC4100TF and incubated at room temperature. Every week two stabs were opened, the bacteria on the top of the medium was removed, diluted and plated in duplicates. Colonies were stained with iodine and counted.Back to article page