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Table 1 Comparison of Protein Expression Profiles during Biofilm and Planktonic Growth

From: Biofilm and planktonic pneumococci demonstrate disparate immunoreactivity to human convalescent sera

    Detected Peptides
Group and Function Protein Gene Biofilm Planktonic
Protein synthesis and processing 30S ribosomal protein S2 rpsB (SP_2215) 14 22
  30S ribosomal protein S3 rpsC (SP_0215) 4 5
  30S ribosomal protein S4 rpsD (SP_0085) 4 5
  30S ribosomal protein S5 rpsE (SP_0227) 6 23
  30S ribosomal protein S8 rpsH (SP_0224) 6 10
  30S ribosomal protein S7 rpsG (SP_0272) 0 3
  30S ribosomal protein S10 rpsJ (SP_0208) 3 4
  30S ribosomal protein S12 rpsL (SP_0271) 0 3
  30S ribosomal protein S11 rpsK (SP_0235) 4 6
  30S ribosomal protein S13 rpsM (SP_0234) 0 2
  30S ribosomal protein S17 rpsQ (SP_0218) 0 4
  50S ribosomal protein L1 rplA (SP_0631) 9 28
  50S ribosomal protein L2 rplB (SP_0212) 0 7
  50S ribosomal protein L3 rplC (SP_0209) 0 4
  50S ribosomal protein L4 rplD (SP_0210) 5 11
  50S ribosomal protein L5 rplE (SP_0221) 10 23
  50S ribosomal protein L6 rplF SP_0225 6 5
  50S ribosomal protein L7/L12 rplL (SP_1354) 0 14
  50S ribosomal protein L9 rplI (SP_2204) 0 2
  50S ribosomal protein L10 rplJ (SP_1355) 0 7
  50S ribosomal protein L11 rplK (SP_0630) 0 9
  50S ribosomal protein L14 rplN (SP_0219) 3 5
  50S ribosomal protein L15 rplO SP_0229 0 5
  50S ribosomal protein L18 rplR (SP_0226) 0 3
  50S ribosomal protein L19 rplS (SP_1293) 8 8
  50S ribosomal protein L21 rplU (SP_1105) 0 2
  50S ribosomal protein L22 rplV (SP_0214) 0 6
  50S ribosomal protein L30 rpmD (SP_0228) 0 2
  Elongation factor G fusA (SP_0273) 10 72
  Elongation factor P efp (SP_0435) 5 7
  Elongation factor Ts tsf (SP_2214) 14 16
  Elongation factor Tu (SP_0681) 29 59
  Arginyl-tRNA synthetase argS (SP_2078) 11 14
  Alanyl-tRNA synthetase alaS (SP_1383) 4 8
  Glutamyl-tRNA(Gln) amidotransferase subunit A gatA (SP_0437) 0 4
  Glycyl-tRNA synthetase alpha subunit glyQ (SP_1475) 0 4
  Methionyl-tRNA formyltransferase fmt (SP_1735) 0 2
  Methionyl-tRNA synthetase metG (SP_0788) 0 2
  Phenylalanyl-tRNA synthetase beta chain pheT (SP_0581) 0 2
  Prolyl-tRNA synthetase proS (SP_0264) 0 12
  10 kDa chaperonin groES (SP_1907) 3 8
  60 kDa chaperonin groEL (SP_1906) 24 57
  33 kDa chaperonin hslO (SP_2188) 0 4
  Chaperone protein dnaK dnaK (SP_0517) 12 89
  ATP-dependent Clp protease ATP-binding subunit clpE (SP_0820) 3 3
  ATP-dependent Clp protease proteolytic subunit clpP (SP_0746) 0 5
  Adapter protein mecA mecA SP_1362 0 2
  Ribosome-recycling factor frr (SP_0945) 0 2
  Foldase protein prsA prsA SP_0981 13 5
  Peptide chain release factor 3 prfC (SP_0439) 4 0
  Peptide deformylase def (SP_1456) 0 4
  Protein grpE grpE (SP_0516) 0 5
Energy Metabolism Phosphoglycerate kinase pgk (SP_0499) 22 46
  L-lactate dehydrogenase ldh (SP_1220) 21 30
  Glyceraldehyde-3-phosphate dehydrogenase gapN (SP_1119) 10 38
  Fructose-bisphosphate aldolase fba SP_0605 6 32
  Glycerol-3-phosphate dehydrogenase [NAD(P)+] gpsA (SP_2091) 2 6
  2,3-bisphosphoglycerate-dependent phosphoglycerate mutase gpmA (SP_1655) 7 11
  6-phosphofructokinase pfkA (SP_0896) 8 21
  Phosphoenolpyruvate-protein phosphotransferase ptsI (SP_1176) 0 10
  Ribose-phosphate pyrophosphokinase 1 prsA (SP_0027) 2 4
  Ribose-5-phosphate isomerase A rpiA (SP_0828) 0 3
  Triosephosphate isomerase tpiA (SP_1574) 2 8
  Tagatose 1,6-diphosphate aldolase lacD (SP_1190) 4 6
  Phosphoenolpyruvate-protein phosphotransferase ptsI (SP_1176) 0 6
  Ribose-phosphate pyrophosphokinase 2 prs2 (SP_1095) 0 3
  Phosphoglucosamine mutase glmM (SP_1559) 0 2
  Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] glmS (SP_0266) 12 31
  Ornithine carbamoyltransferase, catabolic arcB (SP_2150) 4 2
  Dihydrodipicolinate reductase dapB (SP_1555) 0 4
  Dihydrodipicolinate synthase dapA (SP_1014) 0 3
  Glucosamine-6-phosphate deaminase nagB (SP_1415) 0 2
  Carbamoyl-phosphate synthase large chain carB (SP_1275) 5 5
  2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase dapH (SP_2097) 0 8
  Aspartate--ammonia ligase asnA (SP_1970) 3 0
  Dihydroxy-acid dehydratase ilvD (SP_2126) 0 7
  ATP synthase subunit alpha atpA (SP_1510) 4 21
  ATP synthase subunit beta atpD (SP_1508) 9 18
  ATP synthase gamma chain atpG (SP_1509) 0 8
  Phosphate import ATP-binding protein PstB 1 pstB1 (SP_1396) 0 9
  Phosphate import ATP-binding protein PstB 2 pstB2 (SP_1397) 0 4
  Maltose/maltodextrin-binding protein malX (SP_2108) 0 10
  Manganese ABC transporter substrate-binding lipoprotein psaA (SP_1650) 13 0
  GMP synthase [glutamine-hydrolyzing] guaA (SP_1445) 6 21
  Hypoxanthine-guanine phosphoribosyltransferase guaA (SP_1445) 0 9
  Adenylate kinase guaA (SP_1445) 2 5
  Inosine-5'-monophosphate dehydrogenase guaA (SP_1445) 0 14
  Uracil phosphoribosyltransferase guaA (SP_1445) 4 11
  Dihydroorotate dehydrogenase guaA (SP_1445) 0 5
  Uridylate kinase guaA (SP_1445) 0 5
  CTP synthase guaA (SP_1445) 3 0
  Bifunctional protein glmU guaA (SP_1445) 0 3
  Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta accD (SP_0426) 0 2
  Phosphate acyltransferase plsY (SP_0851) 0 2
  Formate--tetrahydrofolate ligase fhs (SP_1229) 0 5
  6,7-dimethyl-8-ribityllumazine synthase ribH (SP_0175) 0 3
  manganese-dependent inorganic pyrophosphatase ppaC (SP_1534) 2 3
  Serine hydroxymethyltransferase glyA (SP_1024) 0 7
  Pyridoxal biosynthesis lyase pdxS pdxS (SP_1468) 0 9
Capsule production & Cell wall Tyrosine-protein kinase CpsD cpsD (SP_0349) 0 2
  Glucan 1,6-alpha-glucosidase dexB (SP_0342) 2 3
  UTP--glucose-1-phosphate uridylyltransferase cap4C (SP_2092) 4 4
  UDP-N-acetylmuramoylalanine--D-glutamate ligase murD (SP_0688) 0 3
  D-alanine--poly(phosphoribitol) ligase subunit 1 dltC (SP_2174) 0 3
Virulence Factors Enolase eno (SP_1128) 25 89
  Pyruvate oxidase spxB (SP_0730) 28 62
  Pneumolysin ply (SP_1923) 0 11
  PsrP psrP (SP_1772) 72 21
Unknown & Hypothetical DegV domain-containing protein (SP_1112) 0 5
  UPF0176 protein (SP_0095) 2 0
  UPF0371 protein (SP_0341) 0 6
  UPF0082 protein (SP_1922) 0 8
  Probable transketolase tkt (SP_2030) 4 37
Regulation & DNA Binding HPr kinase/phosphorylase hprK (SP_1413) 0 2
  Single-stranded DNA-binding protein ssb (SP_1540) 0 9
  DNA-binding protein HU hup (SP_1113) 0 10
  GTP-sensing transcriptional pleiotropic repressor CodY codY (SP_1584) 0 2
  Pur operon repressor purR (SP_1979) 0 5
Transcription DNA-directed RNA polymerase subunit alpha rpoA (SP_0236) 5 3
  DNA-directed RNA polymerase subunit beta rpoB (SP_1961) 2 4
  Transcription elongation factor GreA greA (SP_1517) 0 3