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Figure 4 | BMC Microbiology

Figure 4

From: Development of a gene cloning system in a fast-growing and moderately thermophilic Streptomyces species and heterologous expression of Streptomyces antibiotic biosynthetic gene clusters

Figure 4

Quantitation of actinorhodin production by M145 and by 4F containing the cloned actinorhodin gene cluster in liquid medium. About 1 × 106 spores of M145 and of 4F containing pCWH74 were inoculated into 50 ml R2YE liquid medium (lacking KH2PO4 and CaCl2) at 30 and 37°C. Samples of 1 ml culture were harvested in a time-course and treated with KOH; absorption at OD640 indicated actinorhodin production.

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BMC Microbiology

ISSN: 1471-2180

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