Inhibition of AKT suppresses HSV-1-induced reactivation of KSHV. (A) Western blot analysis was used to detect the expression of KSHV Rta and vIL-6 in AKT-DN or control vector transfected and HSV-1 infected BCBL-1 cells as indicated. (B) KSHV lytic proteins ORF59 expression in AKT-DN or control vector transfected and HSV-1 infected BCBL-1 cells was detected by IFA staining with ORF59 mAb. Original magnifications, × 10. (C) Quantification of results in B. *** P < 0.001 for Student's t-test versus Mock + pSRα group, whereas **P < 0.01 for Student's t-test versus HSV-1 + pSRα group.