Molecular profiling of fungal communities in moisture damaged buildings before and after remediation - a comparison of culture-dependent and culture-independent methods

Table 1 Fungal diversity and concentrations in house dust samples

Sample^a	nucITS clone library analysis^b						Culture	qPCR^c			Erg^d
	N	S_obs	%C	S _ACE	H'	D	total cfu g^-1	S_qPCR	total CE g^-1	ERMI value	μg/g
In1a	225	98	45	220	4.06	0.027	9.6·10⁴	12	1.4·10⁷	4.0	2.6
In1b	100	62	44	142	3.94	0.014	5.7·10³	6	4.4 ·10⁵	-0.7	0.4
Re1a	207	45	44	103	2.22	0.31	2.5·10⁶	9	1.3 ·10⁷	-5.2	5.5
Re1b	26	21	31	67	2.97	0.018	1.4·10²	9	4.0·10⁵	1.0	0.2
In2a	100	37	48	77	2.73	0.148	1.7·10⁶	17	1.2·10⁷	4.4	1.1
In2b	119	42	25	167	2.68	0.186	1.1·10⁶	22	2.6·10⁶	4.3	1.1
Re2a	167	48	52	93	2.95	0.108	1.4·10⁵	10	3.2·10⁷	-1.3	1.9
Re2b	137	75	25	298	3.88	0.030	2.7·10⁵	24	4.1·10⁶	4.6	2.6
Combined data	1081	305	45	675	4.63	0.028		33

^a) Sample name abbreviations: In: index building, Re: reference building, 1: Location-1, 2: Location-2, a: pre-remediation sample, b: post-remediation sample.
^b) Abbreviations: N: number of clones; S_obs: number of observed OTUs; %C: percentage coverage (ACE); S_ACE: total no. of OTUs according to ACE richness estimator; H': Shannon diversity index; D: Simpson diversity index.
^c) Abbreviations: S_qPCR: number of qPCR assays giving positive results; total CE: sum of cell equivalent counts for 69 common indoor fungi, ERMI: Environmental Relative Moldiness Index^.
^d) Concentration of ergosterol.

ISSN: 1471-2180