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Figure 3 | BMC Microbiology

Figure 3

From: Immunodominant proteins α-1 giardin and β-giardin are expressed in both assemblages A and B of Giardia lamblia

Figure 3

Immunolocalization of β-giardin in WB and GS trophozoites. Reactivity of 12G5 mAb on WB and GS Giardia trophozoites was determined by indirect immunofluorescence in permeabilized trophozoites. (A) Upper panel: immunofluorescence assays showing the labelling in the ventral disc of the trophozoites. Lower panels: high magnification showing the immunostaining in the ventral disc of WB trophozoites, with more intensity on the margins. (B) Upper panel: immunofluorescence of β-giardin in GS trophozoites. Lower panels: high magnification showing immunofluorescence specifically organized into a radial array surrounding the half ring of the ventral disc and also at the centre of it. Scale bar: 10 μm.

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