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Table 1 Strains and plasmids used in this work

From: Identification and characterization of PhbF: A DNA binding protein with regulatory role in the PHB metabolism of Herbaspirillum seropedicae SmR1

Strains Relevant genotype Reference/source
E. coli   
BL21(DE3) hsdS gal (λcIts 857 ind1 Sam7 nin5 lacUV5-T7 gene 1). Invitrogen
ET8000 rbs lacZ::IS1 gyrA hutCc k (wild-type). [42]
H. seropedicae   
SmR1 Wild-type, Nif+, SmR. [43]
Plasmids   
pET-28a Expression vector, T7 promoter, KmR. Novagen
pDK6 Expression vector tac promoter lacIq, KmR. [44]
pKADO3 H. seropedicae SmR1 phbF cloned into pET-28a; expresses the His-tag PhbF protein. This work
pKADO5 353 bp containing phbF promoter region cloned into pMP220 resulting in the phbF:: lacZ transcriptional fusion. This work.
pMMS31 Derivative of pDK6 encoding PhbF from H. seropedicae SmR1. This work.
pMMS35 381 bp containing phaP1 promoter region cloned into pMP220 resulting in the phbP1:: lacZ transcriptional fusion. This work.
pMP220 Vector used to construct transcriptional lacZ fusions; TcR. [32]