Table 1 Strains and plasmids used in this work

E. coli

BL21(DE3)

hsdS gal (λcIts 857 ind1 Sam7 nin5 lacUV5-T7 gene 1).

Invitrogen

ET8000

rbs lacZ::IS1 gyrA hutCc k (wild-type).

[42]

H. seropedicae

SmR1

Wild-type, Nif⁺, Sm^R.

[43]

Plasmids

pET-28a

Expression vector, T7 promoter, Km^R.

Novagen

pDK6

Expression vector tac promoter lacIq, Km^R.

[44]

pKADO3

H. seropedicae SmR1 phbF cloned into pET-28a; expresses the His-tag PhbF protein.

This work

pKADO5

353 bp containing phbF promoter region cloned into pMP220 resulting in the phbF:: lacZ transcriptional fusion.

This work.

pMMS31

Derivative of pDK6 encoding PhbF from H. seropedicae SmR1.

This work.

pMMS35

381 bp containing phaP1 promoter region cloned into pMP220 resulting in the phbP1:: lacZ transcriptional fusion.

This work.

pMP220

Vector used to construct transcriptional lacZ fusions; Tc^R.

[32]