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Table 2 Effect of metabolic inhibitors, phosphate analogs, and incubation in the dark on phosphate uptake in Synechocystis sp. PCC 6803a

From: Na+-stimulated phosphate uptake system in Synechocystis sp. PCC 6803 with Pst1 as a main transporter

Treatment Phosphate uptake (%)
Control 100 ± 2
NaF 1 mM 93 ± 5
N, N-dicyclohexylcarbodiimide 40 μMb 91 ± 6
Na+ ionophore 10 μM 91 ± 4
Gramicidin10 μM 80 ± 3
Amiloride 20 μM 77 ± 5
Valinomycin 20 μM 77 ± 4
Monensin 20 μM 69 ± 4
KCN 5 mM 54 ± 3
3-(3, 4-dichlorophenyl)-1, 1-dimethylurea 20 μMb 51 ± 6
Dark 48 ± 5
N-ethylmaleimide 1 mM 31 ± 6
Carbonyl cyanide m-chlorophenylhydrazone 40 μMb 23 ± 6
  1. aCells were preincubated with inhibitors for 30 min before the addition of K2HPO4 to initiate uptake. Data are the mean of three experiments ± SD. bCells were preincubated with inhibitors for 2 min before assays.