Na+-stimulated phosphate uptake system in Synechocystis sp. PCC 6803 with Pst1 as a main transporter

BMC Microbiology

Table 2 Effect of metabolic inhibitors, phosphate analogs, and incubation in the dark on phosphate uptake in Synechocystis sp. PCC 6803^a

Treatment	Phosphate uptake (%)
Control	100 ± 2
NaF 1 mM	93 ± 5
N, N-dicyclohexylcarbodiimide 40 μM^b	91 ± 6
Na⁺ ionophore 10 μM	91 ± 4
Gramicidin10 μM	80 ± 3
Amiloride 20 μM	77 ± 5
Valinomycin 20 μM	77 ± 4
Monensin 20 μM	69 ± 4
KCN 5 mM	54 ± 3
3-(3, 4-dichlorophenyl)-1, 1-dimethylurea 20 μM^b	51 ± 6
Dark	48 ± 5
N-ethylmaleimide 1 mM	31 ± 6
Carbonyl cyanide m-chlorophenylhydrazone 40 μM^b	23 ± 6

^aCells were preincubated with inhibitors for 30 min before the addition of K₂HPO₄ to initiate uptake. Data are the mean of three experiments ± SD. ^bCells were preincubated with inhibitors for 2 min before assays.

ISSN: 1471-2180