Table 2 Primers used for PCR and qPCR
Name | Primer sequence (5'-3') | ||
|---|---|---|---|
Primers for vectors construction | |||
Rgg0182I1 | CTGGAACTGCAGGAGCAGC | ||
Rgg0182I2 | ATAATTTGAGAATTCTGTACCTT | ||
Rgg0182II1 | TCTCTGGAATTCTTTAAATTG C | ||
Rgg0182II2 | CTTGTCCTGCAG TCTCACTCCC | ||
Rgg0182SE1 | CCCCCCCCATATGGGAAAACAAAATGAACTG | ||
Rgg0182SE2 | CCCCGGATCCTTAACCTACAATCGACTTAAA | ||
Pep0182up | GAAGAATTCGTAAAAAGCATCAGATTTTAC | ||
Pep0182down | AACTACTAGTGCATTTTAAGTCGATTGTAGG | ||
SHP0182up | GAAGAATTCAACAATTTCATATGATTTCCTCC | ||
SHP0182down | AACTACTAGTTCAGTCTCTCCTCTTTC | ||
Primers for DNA EMSA | |||
Pldh-5' | ACGCTTTCACTTAATAATTC | ||
Pldh-3' | TGGTCTAAACATCTCCTTA | ||
Pshp-5' | GTAAATACATGTCAATAGGAC | ||
Pshp-3' | TTTGTTTTCCCATATATGCAACC | ||
Ppep-5' | AGCATCAGATTTTACTCCAGATG | ||
Ppep-3' | TTGTAGGTTAATCCCGTTTATGC | ||
Primers for gene expression analysis | |||
Gene | Locus | Forward | Reverse |
ldh | stu1280 | AAGCTATCCTTGACGATGAA | AATAGCAGGTTGACCGATAA |
rgg 0182 | stu0182 | GAAGTGGAGGAGTTGCCTAA | CCCAGCTCTCAATCCCAAA |
hrcA | stu0118 | ACACCTCTTCAAGGAACTGAT | GTCACTTCATCATCGGAGATA |
dnaK | stu0120 | GACATTGACGAAGTCATCCT | GCACCCATAGCAACTACTTC |
dnaJ | stu0121 | CGTGAAGTGACATGTAAGACA | ACCAAGTGGTGTTTGTGTATC |
groES | stu0203 | GAAGGTACCCGTACTCTTACTG | AACGTAATCTTCTCCGTCTTTA |
groEL | stu0204 | ATTGCTTATAATGCCGGTTA | AGCGTTAAATCCTGTACCAA |
hsp33 | stu0180 | TTTAGTAGGTCCTTTCATG | CGATTTCACCAGAAATAAGC |
cspA | stu0837 | ATTGGTTTAACGCTGACAAAG | TAACCTTTTGACCTTCATCGT |
cspB | stu0838 | TATGGCAAATGGAACAGTAAA | CAAGTGATTTGAATCCATCAG |
ctsR | stu0076 | AGATCAGCTCAGCGAACA | AGAATACGCGAACGAATG |
clpC | stu0077 | ACTGGCAGATTATACCAAAGAC | CACCTACCAAGACAGGATTATT |
clpP | stu0356 | CTTGCTCAAGACTCGTAATAACT | AAGCCATATTCAAGTGTTTCTT |
clpX | stu0581 | TGGACTTATCCCTGAATTTATC | AAGACAAGAGGGTTTGATACTG |
clpE | stu0602 | CCGTACCAAGAACAATCCT | ACGGATAACTTGCTTGCTT |
clpL | stu1614 | CGTTTCGACGCAGTTATT | TTCGCTAACTGCCAAGTC |