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Table 2 Correlation between the lesion diameters of the fruit samples, the amount of B. cinerea antigen determinated by the ELISA developed and the DNA of B. cinerea quantified from infected fruit extracts samples obtained at 4, 7, and 10 days of incubation (25°C), respectively.

From: Development of an indirect competitive enzyme-linked immunosorbent assay applied to the Botrytis cinerea quantification in tissues of postharvest fruits

Fruit samples

Days of incubation

bLesion diameters (mm/rot)

c B. cinereaantigen

(μg mL-1)

c DNA- B. cinerea

(μg mL-1)

Apples (Red-delicious)

aControl

uninfected

not detected

not detected

 

4

not visible

10.53 ± 0.48

10.22 ± 0.53

 

7

20.11 ± 0.54

40.67 ± 0.37

38.75 ± 0.41

 

10

50.09 ± 4.49

69.08 ± 0.43

71.19 ± 0.37

Table grapes (pink Moscatel)

aControl

uninfected

not detected

not detected

 

4

not visible

14.26 ± 0.51

13.86 ± 0.54

 

7

3.69 ± 0.52

49.03 ± 0.46

51.99 ± 0.42

 

10

5.35 ± 0.14

77.18 ± 0.36

75.84 ± 0.41

Pears (William's)

aControl

uninfected

not detected

not detected

 

4

not visible

11.29 ± 0.47

12.76 ± 0.51

 

7

15.13 ± 1.23

41.78 ± 0.55

41.44 ± 0.48

 

10

38.98 ± 1.67

70.84 ± 0.49

72.39 ± 0.52

  1. a Negative control (uninfected fruits).
  2. b Diameters of the lesion measured in the fruit samples at 4, 7 and 10 days of incubation (25°C) respectively.
  3. b, c X (μg mL-1), mean ± SD, standard deviation.