Figure 4

The effect of phosphate and pH on the expression of pyoverdin-related genes. (A, A') Transcriptional pattern response of P. aeruginosa PAO1 to phosphate limitation (< 0.1 mM) displayed at different scales: (A) in the absence of phosphate-related genes and (A') in the presence of phosphate-related genes. Pattern was drawn based on the results of Zaborin et al., 2009. (B) Transcriptional pattern response of P. aeruginosa PAO1 to a pH shift from 6.0 to 7.5 during phosphate sufficiency (25 mM). Pattern was drawn based on the current data. (C) Transcriptional response of IS (mainly pyoverdin-related genes) and FUR regulon in P. aeruginosa PAO1 during iron limitation. Pattern was drawn based on the results of Ochsner et al., 2002. Light green dots represent the fold expression in pyoverdin-related genes; dark green dots - FUR-regulated genes. The dark green circle surrounding pvdS indicates that this gene is regulated by FUR. The brown spots indicate genes involved in pyocyanin biosynthesis, red spots indicate genes belonging to MvfR and MvfR-regulated pqsABCDE operon, and pink spots indicate genes of quorum sensing regulatory elements such as rhlI, rhlR, lasI, lasR, gacA, vfR, qscR. The dark circle surrounding qscR indicates that this gene is involved in the regulation of pyocyanin biosynthesis. Blue spots in the panel A' represent phosphate-related genes.