Figure 2

AFP _NN5353 susceptibility of A. nidulans mutants RhoA^G14V, RhoA^E40I, alcA -PkcA and Δ mpkA compared to the respective recipient strains GR5 and R153. (A) A total of 2 × 10³ conidia were point inoculated on agar plates (CM for GR5, RhoA^G14V, RhoA^E40I and ΔmpkA, repressive MM containing 1% glucose according to [26] for R135 and alcA-PkcA) containing the appropriate supplements and 0, 0.2 and 1 μg/ml AFP_NN5353 for GR5, RhoA^G14V, RhoA^E40I, R135 and alcA-PkcA. The ΔmpkA mutant and its reference strain GR5 were exposed to 0, 0.5 and 1 μg/ml AFP_NN5353. The plates were incubated at 37°C for 48 h. (B) 1 × 10⁴ conidia/ml of the ΔmpkA mutant and GR5 were treated with 0.05 μg/ml AFP_NN5353 or without protein (controls) in a total volume of 200 μl of appropriately supplemented CM in 96-well plates.